<p><strong>Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “</strong><i><strong>Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA</strong></i><strong>”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.</strong></p><h5><strong>Could you describe your project and its main objectives?</strong></h5><p>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.</p><p>Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.</p><h5><strong>Why is this export of unspliced ​​RNA so important for retroviral replication?</strong></h5><p>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.</p><p><strong>Simply put: </strong>the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.</p><h5><strong>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</strong></h5><p>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the<strong> NIH </strong>and <strong>Rockefeller University</strong>, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.</p><h5><strong>How will this research contribute to the development of new treatments for retroviral infections?</strong></h5><p>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.</p><h5><strong>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</strong></h5><p>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from <strong>NIH, Frederick</strong>, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from <strong>Rockefeller University to employ iCLIP </strong>methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.</p><h5><strong>Who else is collaborating on the project?</strong></h5><p>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of<strong> Organic Chemistry and Biochemistry of the CAS (IOCB Prague)</strong> and the <strong>Central European Institute of Technology in Brno (CEITEC)</strong>. Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.</p><h5><strong>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</strong></h5><p>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.</p><h5><strong>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</strong></h5><p>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.</p>

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

<p><strong>🎤Lindsay Repka&nbsp;</strong></p><p><strong>Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.</strong></p><p>In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.</p><p>What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.</p><p>This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.</p><p><strong>If you work in:</strong></p><ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul><p>…this conversation will resonate.</p><h4><strong>Key Topics Covered</strong></h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3><strong>Video Transcription</strong></h3><p>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.</p><h4><strong>What is photoredox chemistry?</strong></h4><p>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.</p><p>Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.</p><h4><strong>Photocrosslinking and drug-target interactions</strong></h4><p>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.</p><p>Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.</p><h4><strong>From photocrosslinking to solvent activation</strong></h4><p>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.</p><p>The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.</p><h4><strong>Why LC-MS was critical</strong></h4><p>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.</p><p>Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.</p><h4><strong>Photocatalyst stability workflow</strong></h4><p>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.</p><p>Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.</p><h4><strong>Quantifying catalyst remaining by LC-MS</strong></h4><p>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.</p><p>Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.</p><p>Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.</p><h4><strong>Why GC-MS was used later</strong></h4><p>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.</p><p>Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.</p><h4><strong>Practical lessons from the project</strong></h4><p>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.</p><p>She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.</p><h4><strong>Final perspective</strong></h4><p>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

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The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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<header><p><strong>Join Us for an Engaging Live Webinar on Integrating UV/Vis Spectroscopy Into Your Laboratory Workflows</strong></p></header><main><div><div><p><strong>Program&nbsp;</strong></p><ul><li>Introduction to UV/Vis Spectroscopy</li><li>The Power of Integration</li><li>Live Demonstration</li><li>Customer Use Cases</li><li>Engage &amp; Discuss</li></ul></div></div><div><div><p>Discover how connecting your UV/Vis spectrophotometer with instruments like balances can transform your laboratory processes. By transmitting dissolution data directly to the spectrophotometer, you can streamline workflows and enhance data quality, leading to improved productivity and accuracy.</p></div></div><div><div><div><p>In this informative session, we’ll showcase the power of instrument integration and how it can revolutionize your daily routines. Using real-world case studies from various industries, including battery manufacturing, pharmaceuticals, and food and beverage, you’ll gain valuable insights into optimizing your laboratory workflows.</p><p><strong>Program Highlights</strong></p><ul><li><strong>Introduction to UV/Vis Spectroscopy</strong><br>Understand the basics and applications of UV/Vis spectroscopy in laboratories.</li><li><strong>The Power of Integration</strong><br>Explore the benefits of connecting your UV/Vis spectrophotometer with balances and other instruments.</li><li><strong>Live Demonstration</strong><br>Witness a live demonstration of data transmission from a balance to a UV/Vis spectrophotometer.</li><li><strong>Customer Use Cases</strong><br>Learn about integrated processes used in the battery, pharma, and food &amp; beverage industries.</li><li><strong>Engage &amp; Discuss</strong><br>Participate in a Q&amp;A session where we address your questions and discuss potential applications in your own lab.</li></ul><p>Don’t miss this opportunity to explore innovative solutions that can help you think critically about your own processes and enhance your laboratory's efficiency!</p></div></div></div></main><div><div><p><strong>Target Audience</strong></p><div><p>This webinar is designed for laboratory professionals, scientists, and managers across various industries, including:</p><ul><li>Pharmaceuticals: Quality control and research &amp; development teams looking to improve analytical processes</li><li>Food &amp; Beverage: Quality assurance and product development personnel aiming to enhance product testing efficiency</li><li>Battery Manufacturing: Engineers and technicians interested in optimizing material testing workflows</li><li>Laboratory Managers: Professionals seeking to streamline operations and improve data quality through instrument integration</li></ul><p>Whether you are just starting with UV/Vis spectroscopy or looking to enhance your existing laboratory workflows, this webinar is perfect for you. Register now to secure your spot and take the first step towards a more efficient laboratory.</p><ul><li><span style="padding-right:0px;" id="webinar-session-1"><strong>1. 10. 2025 9:00,&nbsp; English&nbsp;</strong></span><span id="webinar-session-2"><strong><svg width="16" height="16" viewBox="0 0 16 16" fill="none" xmlns="http://www.w3.org/2000/svg"></svg></strong></span></li><li><span id="webinar-session-2"><strong>1. 10. 2025 17:00,&nbsp; English&nbsp;</strong></span></li></ul><blockquote><p><span id="webinar-session-2"><strong>Presenter: </strong></span>Anika Adams (Product Manager, SPG UV/VIS)</p><div><p>Anika Adams is Product Manager for UV/Vis Spectrophotometers at METTLER TOLEDO, where she focuses on driving innovation and optimizing customer workflows. With a degree in business administration and experience spanning software development, mechanical engineering, and production, Anika brings a multidisciplinary perspective to the laboratory business. Her expertise lies in automating and enhancing customer processes, ensuring both product excellence and customer satisfaction.</p><p><strong>Presenter:</strong> Dr. Fabian Müller (Product Manager, SPG UV/VIS)</p><div><div><div><div><p>Dr. Fabian Müller, Ph.D., is an accomplished chemist from the ETH Zürich in Switzerland. After completing his doctoral studies, Dr. Müller joined the team at METTLER TOLEDO as an Application Specialist for UV/Vis spectroscopy, where he quickly established himself as a skilled and knowledgeable professional. His expertise in this area led to his promotion to the position of Product Manager for UV/Vis spectrophotometers.</p></div></div></div></div></div></blockquote></div></div></div>

Mettler Toledo: UV/Vis Integration in Laboratory Workflows 

UV/Vis Integration in Laboratory Workflows 

Join Us for an Engaging Live Webinar on Integrating UV/Vis Spectroscopy Into Your Laboratory Workflows

<div><h4><strong>Start Your Journey to ASTRA Expertise</strong></h4><p>Join us for the last <strong>5 series</strong> on building the perfect method for light scattering analysis.</p><p>Learn advanced tools and techniques to master ASTRA software, from normalization to DLS with flowing systems.</p><h4><strong>Session Overview:</strong></h4><ul><li><strong>Part 6 of 10</strong> Baselines &amp; Peaks - Best Practices &amp; Navigating Unexpected Chromatograms<ul><li>Thursday, September 11</li></ul></li><li><strong>Part 7 of 10 </strong>Light Scattering Formalisms - Zimm, Mie, Berry, Debye, oh my! When and Why to Use Them<ul><li>Thursday, September 25</li></ul></li><li><h5><strong>Part 8 of 10: Calculation Uncertainty and Error Analysis: Establishing Meaningful MALS Results</strong></h5><ul><li>Thursday, October 2</li></ul></li><li><strong>Part 9 of 10: </strong>Distribution Analysis &amp; Results Fitting: Advanced Peak Analysis Tools<ul><li>Thursday, October 9</li></ul></li><li><strong>Part 10 of 10:</strong> Mass Recovery &amp; Data Exporting: Effective Reporting of Results and Findings<ul><li>Thursday, October 23</li></ul></li></ul><h4><strong>Why Attend:</strong></h4><ul><li>Deepen your understanding of light scattering and ASTRA.</li><li>Gain actionable insights for your lab.</li><li>Interact with our expert, Dr. Christian Ackerschott.<br>&nbsp;</li></ul><blockquote><p><strong>Hosted by:</strong> Dr. Christian Ackerschott</p><p>Dr. Christian Ackerschott studied Chemistry at the University of Cologne and finished with his diploma thesis in Biochemistry. He completed his studies with the PhD thesis “Characterization of Recombinant Flagelliform Spider Silk Proteins” at the Technische Universität München, where AF4-MALS was used as an essential characterization method.</p><p>Since 2007, Christian works at Wyatt Technology as Application Scientist and is now Head of Customer Service. His tasks also include customer trainings in Dernbach and onsite as well as education and training of the local Field Application Scientists.</p></blockquote></div>

Waters Corporation: Interactive Web Series EU – Becoming a SEC-MALS & ASTRA Expert

Becoming a SEC-MALS & ASTRA Expert: Part 8

Improve the reliability of your results by understanding error sources and uncertainty in MALS. Learn how to establish meaningful conclusions.

Becoming SEC-MALS & ASTRA Expert Part 8

<div><p><strong>Join us for the last 5 series on building the perfect method for light scattering analysis.</strong></p><p><strong>Learn advanced tools and techniques to master ASTRA software, from normalization to DLS with flowing systems.</strong></p><h4><strong>Session Overview:</strong></h4><ul><li><strong>Part 6 of 10: </strong>Building the Perfect Method - Normalization, Alignment, &amp; Inter-Detector Band Broadening Corrections<ul><li>Friday, September 26</li></ul></li><li><h5><strong>Part 7 of 10: Navigating Sample Parameters – A Close Look at dn/dc and Extinction Coefficients</strong></h5><ul><li>Friday, October 3</li></ul></li><li><strong>Part 8 of 10: </strong>Advanced Characterization Tools for Multi-Component Analytes - Protein Conjugates, AAV, LNP and ADC<ul><li>Friday, October 10</li></ul></li><li><strong>Part 9 of 10: </strong>ASTRA for Particles: When and How to Deploy<ul><li>Friday, October 17</li></ul></li><li><strong>Part 10 of 10: </strong>DLS with Flowing Systems – Leveraging Rh and the Burchard-Stockmayer Plot in ASTRA<ul><li>Friday, October 24</li></ul></li></ul><h4><strong>Why Attend:</strong></h4><ul><li>Deepen your understanding of light scattering and ASTRA.</li><li>Gain actionable insights for your lab.</li><li>Interact with our expert, Dr. Andy Meyer.</li></ul><p>The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all Wyatt customers.</p><blockquote><p><strong>Hosted by:</strong> Dr. Andy Meyer</p><p>Dr. Andy Meyer joined Wyatt Technology in 2001, serving for several years as an Applications Scientist, then as Dean of Wyatt's "Light Scattering University" and as Director of Customer Service &amp; Support. After managing multiple regions in North America, Dr. Meyer is now a Principal Trainer for Waters | Wyatt Technology. As a Wyatt customer, he completed his Ph.D. in Applied Science – Polymer Chemistry from the College of William and Mary in Virginia.</p></blockquote></div>

Waters Corporation: Becoming an ASTRA Expert

Becoming an ASTRA Expert: Part 7

Master the use of dn/dc and extinction coefficients in ASTRA. Understand how these key parameters influence accurate molar mass and concentration results.

<p><strong>GLP-1 receptor agonists like Semaglutide and Tirzepatide are complex therapeutic peptides used in treating type 2 diabetes and obesity. Their structural complexity and formulation with diverse excipients pose significant analytical challenges. This webinar will explore impurity profiling and excipient analysis using orthogonal HILIC-UV/MS and HILIC-ELSD workflows.</strong></p><p>Attendees will gain insights into mitigating nonspecific adsorption issues and improving method robustness using low-adsorption LC flow paths. The session will also highlight how these workflows complement traditional methods and support regulatory compliance through Analytical Quality by Design (AQbD) principles.</p><h5><strong>Key Learning Objectives:</strong></h5><ul><li>Understand the structural and analytical challenges of GLP-1 receptor agonists.</li><li>Learn how HILIC-UV/MS and HILIC-ELSD workflows enhance impurity profiling.</li><li>Discover strategies to reduce nonspecific adsorption and improve recovery.</li></ul><h5><strong>Who Should Attend:</strong></h5><ul><li>Laboratory Managers</li><li>Chromatographers</li><li>Biopharmaceutical Scientists</li><li>Analytical Chemists</li></ul><blockquote><p><strong>Presenter: </strong>Amanda Guiraldelli Mahr, Ph.D. (Senior Scientist, Proteins Chemistry and Separation, RIC Group, Belgium)</p></blockquote>

C&EN: Advanced HILIC Workflows for GLP-1 Agonist Impurity Profiling

Advanced HILIC Workflows for GLP-1 Agonist Impurity Profiling - Session 2

Explore HILIC-based impurity analysis of GLP-1 agonists. Improve robustness and recovery using low-adsorption LC paths for complex peptide formulations.

HILIC Workflows for GLP-1 Agonist Impurity Profiling

<div><p><strong>Analytical methods are essential to ensuring pharmaceutical product quality, regulatory compliance, and patient safety. However, their reliability and reproducibility can be compromised by multiple sources of variability—including reagent quality, instrument performance, operator technique, sample preparation, and data processing. Understanding and controlling these sources is critical, especially when justifying post-approval changes to validated methods.</strong></p><p>This three-part webinar series features expert Peter Baker (Live Oak QA, Austin, Texas) and focuses on the importance of identifying, monitoring, and documenting variability within analytical workflows. Through open discussion and real-world insights, participants will gain a deeper understanding of how to build a scientifically sound case for method adjustments while maintaining regulatory compliance. Each session includes a live Q&amp;A to encourage interaction and address specific challenges.</p><h4><strong>Session 3: Analytical Control in Action – Real-World Case Studies</strong></h4><p>The final session presents real-world examples of method variability and how companies have successfully justified changes due to shifts in materials, instruments, or processes. Emphasis will be placed on documentation, regulatory communication, and lessons learned.</p><p><i><strong>Goal: </strong>Apply insights from case studies to your own workflows and gain practical strategies for managing change.</i><br>&nbsp;</p><blockquote><p><strong>Presenter:</strong> Peter Baker (President, Live Oak Quality Assurance LLC)</p><p>Peter E. Baker, President, Live Oak Quality Assurance LLC, has been consulting nationally and internationally since April 2019. Prior to consulting, Peter spent 11 years as a U.S. FDA Drug Investigator, with most of those years spent working in FDA’s overseas offices located in India, China and Chile. Peter was named FDA Investigator of the Year in 2013 for his work uncovering serious breaches in data integrity and has special interest in data governance and compliance with 21 CFR Part 11. Live Oak focuses on training and employee empowerment in critical thinking, with the goal of improving the quality of medicines through good data management.</p><p><strong>Presenter: </strong>Dr. Stephanie Harden (Senior Manager, Small Molecule Core Solutions – LC-MS, Waters Corporation)</p><p>Stephanie Harden, Senior Manager at Waters Corporation, leads the Product Marketing and Scientific teams for Waters small molecule HPLC portfolio. With over 25 years of experience in product and segment marketing, she has a proven track record in strategic program development, cross-functional leadership, and market-driven innovation. Her work connects scientific insight with commercial strategy to drive customer-focused solutions and sustained business growth. Stephanie holds a PhD in Chemistry from the University of Bristol, UK, and is a widely cited author.</p></blockquote></div>

Waters Corporation: Managing Method Variability: A Foundation for Risk-Based Change

Managing Method Variability: A Foundation for Risk-Based Change - Session 3

Discover real case studies on managing analytical variability, documenting changes, and maintaining compliance in pharma workflows.

Analytical Control in Action – Real-World Case Studies

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>As global food safety regulations become increasingly stringent and consumer scrutiny intensifies, laboratories are under pressure to deliver rapid, accurate results while managing complex sample matrices.</strong></p><p>Analyzing emerging contaminants such as PFAS, pesticides, veterinary drugs and other residues in processed or high-fat foods poses significant challenges in method robustness, instrument uptime and data reliability.</p><p>This webinar will explore how the QSight 500 LC-MS/MS is addressing these evolving demands to provide high-quality data for even the most demanding food matrices.</p><h5><strong>Attend this webinar to:</strong></h5><ul><li>Gain insights into how to optimize your LC-MS/MS workflows for better efficiency and confidence in your results</li><li>Understand how to reliably detect contaminants across complex matrices&nbsp;</li><li>Learn how the QSight 500 LC-MS/MS system brings greater operational efficiency with reduced instrument cleaning&nbsp;</li><li>Explore how enhancing system robustness reduces cost per sample&nbsp;</li></ul><blockquote><p><strong>Presenter: </strong>Jamie Foss (Product Manager, Liquid Chromatography &amp; Mass Spectrometry at PerkinElmer)</p><p>Jamie Foss is the Liquid Chromatography and Mass Spectrometry Product Manager at PerkinElmer. Prior to this role, he was a Sr. Application Scientist developing strategic applications in support PerkinElmer’s LC and LC/MS product portfolio. He has developed a wide variety of applications across food, environmental, industrial, forensics and cannabis. Prior to joining PerkinElmer in 2016, he spent 4 years as a forensic chemist for the State of Maine where he focused on the analysis of controlled substances and clandestine laboratory investigation.</p></blockquote></div></div></div>

Technology Networks: Addressing Evolving Demands in Food Analysis

Addressing Evolving Demands in Food Analysis

Optimize food contaminant analysis with QSight 500 LC-MS/MS. Robust, efficient workflows for PFAS, pesticides, and residues in demanding food matrices.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Dr. Hale's research integrates native ambient mass spectrometry (NAMS) to analyze intact proteoform complexes in tissue samples under non-denaturing conditions. Utilizing nano-DESI (nano spray desorption electrospray ionization), this technique enables the spatial mapping of proteoforms within tissues, revealing their distribution and functional implications. The method involves home-built ion sources that provide high-resolution imaging compatible with advanced mass spectrometers like the Orbitrap Eclipse.</strong></p><p>The presentation highlights the ability to analyze proteoforms and their post-translational modifications (PTMs) directly from tissue samples without extensive preparation, preserving the native state of protein complexes. This approach is particularly useful for studying neurodegenerative diseases, where understanding the spatial distribution and interactions of proteoforms can provide insights into disease mechanisms. Dr. Hale emphasizes the importance of this technology in advancing pathology research and improving the detection and identification of high molecular weight proteins and membrane protein complexes.</p><h5><strong>Learning points:</strong></h5><ul><li><strong>Spatial Distribution Analysis: </strong>Nano-DESI mass spectrometry enables the spatial mapping of proteoforms within tissues, providing insights into their distribution and functional roles, which is crucial for understanding disease mechanisms, particularly in neurodegenerative diseases.&nbsp;</li><li><strong>Non-Denaturing Conditions: </strong>The technique preserves the native state of protein complexes during analysis, allowing for the study of intact proteoforms and their post-translational modifications (PTMs) directly from tissue samples without extensive preparation.&nbsp;</li><li><strong>Advanced Mass Spectrometry Techniques: </strong>Utilizing high-resolution mass spectrometers like the Orbitrap Eclipse, the method allows for detailed analysis of high molecular weight proteins and membrane protein complexes, enhancing the detection and identification capabilities for complex biological samples</li></ul><h5><strong>Who should attend:</strong></h5><ul><li><strong>Pathology Researchers: </strong>This technique provides valuable insights into the spatial distribution and interactions of proteoforms within tissues, aiding in the understanding of disease mechanisms and pathology.&nbsp;</li><li><strong>Neurodegenerative Disease Scientists: </strong>The ability to analyze intact proteoform complexes in their native state is particularly beneficial for studying neurodegenerative diseases, offering potential breakthroughs in understanding disease progression and identifying therapeutic targets.&nbsp;</li><li><strong>Mass Spectrometry Technologists:</strong> Professionals working with advanced mass spectrometry techniques can benefit from the innovative application of nano-DESI for high-resolution imaging, enhancing their analytical capabilities and methodologies for complex biological samples. &nbsp;</li></ul><p>If you cannot attend this webinar please register to receive a link to the On Demand version the following day.&nbsp;</p><blockquote><p><strong>Presenter: </strong>Dr. Oliver Hale (Postdoctoral Research Fellow, University of Birmingham)</p><p>Dr Oliver Hale is a postdoctoral research fellow who specializes in mass spectrometry. His current focus is on combing native mass spectrometry analysis of intact proteins and mass spectrometry imaging, as part of the EPSRC-funded NAMS project.</p></blockquote></div></div></div>

Thermo Fisher Scientific: ASMS 2025 Workshops

Imaging of intact proteoform complexes in tissue

Learn how nano-DESI native ambient MS maps proteoforms in tissues under non-denaturing conditions, advancing insights into neurodegeneration and pathology.

<p><strong>Gel permeation chromatography (GPC), also known as size exclusion chromatography (SEC), is a critical technique for determining the molecular weight and structural parameters of polymers. While application of this technique spans a wide range of industries from advanced materials to pharmaceuticals, scientists often face significant challenges in method development, data interpretation, and troubleshooting. These complexities can compromise results and slow down your research.</strong></p><p>Join our "Ask the Expert" session for a unique opportunity to get personalized guidance from Agilent GPC specialists. Bring your specific questions and challenges, and our experts will provide practical insights to help you get the most out of your instrumentation.</p><h5><strong>This interactive session will focus on key topics, including:</strong></h5><ul><li><strong>Method Development:</strong> Best practices for system configuration and robust method development.</li><li><strong>Component Selection: </strong>How to choose the right columns, standards, and detectors for your application.</li><li><strong>Advanced Setups: </strong>Insights into multi-detector systems (RI, UV, MALS, viscometry).</li><li><strong>Data Analysis: </strong>Utilizing software tools for accurate and reliable data interpretation.</li><li><strong>Troubleshooting: </strong>Strategies to overcome common GPC issues.</li></ul><p>Register now to submit your questions, and we will answer them live during the session.</p><blockquote><p><strong>Presenter:&nbsp;</strong> Dr. Andreas Tei (Global Marketing Product Manager Small Molecules LC &amp; GPC Columns, Agilent Technologies)</p><p>Andreas holds master’s and PhD degrees in synthetic organic chemistry from the University of Heidelberg in Germany. He has almost 30 years of experience in analytical and preparative chromatography, starting as an instrument operator for GC/MS and LC/MS instrumentation at the Institute of Pharmacy at the University of Heidelberg. Andreas joined Agilent as a product manager for LC instrumentation, participating in the development of Agilent’s Automated Purification software and Infinity II LC instrumentation. He is now a marketing product manager in Agilent’s small molecule LC and GPC columns and calibration standards portfolio and is involved in creating the next generation of GPC consumables.</p><p><strong>Presenter: </strong>Mr. Ganesh Kokate (Pre-Sales Application Support, Agilent Technologies)</p><p>Ganesh is a seasoned pre-sales application engineer with over 24 years of experience in industrial and analytical development roles. Holding a master’s degree in organic chemistry, Ganesh began his career at USV and has since contributed to leading pharmaceutical organizations including Cipla, HIKAL, Torrent, and TCG Lifesciences. He has been at Agilent for three years, specializing in GPC, HPLC method development, prep HPLC, LC/MS, and NMR. His work focuses on impurity profiling of new drug molecules, combinatorial compounds, and biological samples, leveraging chromatography and spectroscopy to determine chemical and physical properties, composition, structure, and reactions of organic and inorganic compounds.</p><p><strong>Presenter: </strong>Dr. Ramaprasad Ganni (Technical Support Specialist, Agilent Technologies)</p><p>Ramaprasad has 21 years of experience in the field of chromatography and column chemistries. Having previously worked for Elsevier, Germany, and Millipore, India, he joined Agilent Technologies in 2008 in the Chemistries and Supplies division, where he is responsible for workflow solutions development, providing technical support, and trainings for the food, pharma, petroleum, and biotechnology sectors. His experience spans application development, LC and GC column chemistries and technologies, sample preparation techniques, method development, and troubleshooting issues in LC, GC, mass spectrometry, GPC/SEC, and preparative LC.</p><p><strong>Presenter: </strong>Mr. Shreyas Tribhuvane (Business Development Manager, LC Columns, Agilent Technologies)</p><p>Shreyas has 10 years of experience working closely with biopharma customers to understand their analytical characterization needs. As a business development manager for biocolumns and glycan kits, his primary responsibility focuses on assessing the needs of the biopharma market and communicating with the Agilent solutions unit team in order to deliver optimized solutions. He uses his hands-on experience with Agilent glycan sample prep kits to help customers use these kits to optimize their glycan characterization work.</p></blockquote>

Agilent Technologies: Ask the Expert: Master Your GPC Workflow

Ask the Expert: Master Your GPC Workflow

Agilent experts share best practices in GPC/SEC method development, detector setups, and troubleshooting. Join the interactive session.

<h4><strong>Are you navigating ICH M10 requirements for your regulated bioanalysis workflows?</strong></h4><p>Join our experts as we break down the globally harmonized ICH M10 guidelines, show how labs are applying them in practice, and explore how SCIEX OS software helps ensure quality, efficiency, and compliance.</p><h5><strong>Key highlights:</strong></h5><ul><li>What the ICH M10 guideline is and why it matters for regulated bioanalysis</li><li>How pharmaceutical labs are approaching method validation and study sample analysis</li><li>Common workflow challenges and inefficiencies in meeting regulatory expectations</li><li>How SCIEX OS software simplifies data management, calculations, and reporting</li><li>A live demonstration of current calculation workflows using SCIEX OS software</li><li>A vision for the future of streamlined bioanalytical validation</li></ul><blockquote><p><strong>Presenter: </strong>Henri Meijering, Ardena</p><p>Henri Meijering is a seasoned bioanalytical scientist with over 20 years of experience in drug development and pharmacokinetics. He specializes in developing and validating LC-MS/MS and HPLC methods for both small and large molecules. At Ardena Bioanalytical Laboratory, Henri leads scientific strategy and supports complex studies as Business Support Manager. A former core member of the EBF during the ICH M10 rollout, he is passionate about advancing scientific rigor and compliance in regulated bioanalysis.</p><p><br><strong>Presenter: </strong>Heather Hughes, SCIEX</p><p>Heather Hughes holds a degree in chemistry from Durham University and has built a career spanning clinical and pharmaceutical science. They began in NHS hospital laboratories, specializing in clinical chemistry, before moving into the pharmaceutical industry with experience at Pfizer and a contract research organization. With a background in both regulated and non-regulated environments, they joined SCIEX nine years ago as an application specialist. In this role, they support customers through training, troubleshooting, and showcasing SCIEX mass spectrometry solutions for small molecule workflows.</p></blockquote>

Streamline your ICH M10 workflows

Webinar on ICH M10 bioanalysis guidelines—learn method validation, sample analysis, and how SCIEX OS software ensures compliance and efficiency.

<p><strong>Food and environmental laboratories and contract testing labs are challenged with high sample volumes, regulatory requirements, and the need for consistent data integrity. A digital platform that can centralize workflow management, instrument connectivity, and compliance tracking is becoming essential.</strong></p><p>Agilent SLIMS unifies sample management, workflow execution, consumables tracking, and reporting into a single platform, providing labs with full visibility and control. This webinar session will demonstrate how SLIMS supports food and environmental workflows by ensuring compliance with ISO 17025, improving operational efficiency, and maintaining data traceability. Workflow examples, ranging from pesticide analysis to PFAS testing, will illustrate how SLIMS can be adapted to diverse applications.</p><h5><strong>Who Should Attend:</strong></h5><ul><li>Food and environmental contract testing lab managers</li><li>Quality assurance and compliance teams working under ISO 17025 standards</li><li>Laboratory operations leads handling multi-step sample workflows</li><li>Scientists and technical staff involved in data management and reporting</li><li>IT/LIMS administrators responsible for digital lab transformation</li></ul><h6><strong>Learning Outcomes:</strong></h6><ul><li>Attendees will gain clarity on how SLIMS can:</li><li>Centralize the management of food and environmental testing workflows from sample intake to final reporting</li><li>Integrate instrument data, consumables inventory, digital SOPs, and chain of custody in one system</li><li>Support ISO 17025 compliance through controlled processes, audit trails, and digital documentation</li><li>Provide adaptable workflows across applications such as pesticides, heavy metals, and PFAS, demonstrating flexibility for diverse analytical needs</li><li>Deliver practical improvements in turnaround times, operational consistency, and reporting reliability across contract testing labs</li></ul><blockquote><p><strong>Presenter:&nbsp;</strong> Ralf Davidsson (Product Specialist, Lab Informatics, Agilent Technologies, Australia)</p><p>Based in Brisbane, Australia, Ralf has been with Agilent Technologies for 19 years, and for the past 9 years as a Lab Informatics Product Specialist.</p><p>Holding a MSc degree in Chemical Engineering, he has previously worked in the pharmaceutical industry with method development and validation.</p><p>In his current role, he supports the sales teams and customers with the solution design of digital lab solutions.</p></blockquote>

Agilent Technologies: Every Sample Counts: How SLIMS Transforms Testing Workflows

Every Sample Counts: How SLIMS Transforms Testing Workflows

Webinar on Agilent SLIMS: unify workflows, ensure ISO 17025 compliance, and boost efficiency in food and environmental contract testing labs.