<div><p><strong>Protein purification is a critical step in biopharmaceutical research, proteomics, and industrial biotechnology. It involves isolating a specific protein from complex biological mixtures while preserving its native structure and activity. Traditional methods such as precipitation or dialysis lack the precision required for high-purity protein isolation, especially when dealing with therapeutic targets or complex protein assemblies.</strong></p><p>High-performance liquid chromatography (HPLC) protein purification has transformed this field by providing high resolution, reproducibility, and speed, making it indispensable for both analytical and preparative workflows.</p></div><div><div><div><div><h3><strong>What is HPLC Protein Purification?</strong></h3><p>HPLC purification is based on the principle of selective interactions between proteins and the stationary phase under high-pressure liquid flow. The separation relies on differences in size, charge, hydrophobicity, or affinity of proteins toward the stationary phase material.</p><p>Unlike conventional low-pressure methods such as gel filtration or affinity batch purification, HPLC purification operates under high pressure (up to 6000 psi), enabling faster separation with enhanced resolution and quantitative recovery. It also offers precise control over parameters like gradient composition, pH, and temperature, minimizing variability between runs.</p><p>In contrast to traditional column chromatography, HPLC uses smaller stationary phase particles (3–10 µm), resulting in increased surface area and sharper <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/how-to-reduce-peak-tailing-in-hplc"><strong>peak shapes</strong></a>, which are vital for protein purity assessment and scale-up purification.</p></div></div></div></div><div><div><div><div><h3><strong>Types of HPLC Used for Protein Purification</strong></h3></div></div></div></div><div><p>Different HPLC modes can be employed depending on the physicochemical properties of the target protein. Each method offers unique advantages in terms of selectivity and recovery.</p><h4><strong>Reversed-Phase HPLC (RP-HPLC)</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase"><strong>Reversed-Phase HPLC</strong></a> separates proteins based on hydrophobic interactions between the analyte and the stationary phase, typically C4, C8, or C18 silica-based columns. Proteins are eluted using gradients of organic solvents (usually acetonitrile or methanol) in the presence of volatile acids like formic or trifluoroacetic acid (TFA).</p><p>This method provides exceptional resolution and is commonly used for protein fragment purification, and analysis of recombinant proteins. For example, reversed-phase HPLC is routinely employed in peptide therapeutic workflows, such as the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an59280424_w_fc6c3ac6f0.pdf"><strong> Tirzepatide Preparation and Purification </strong></a>process, where purity and recovery are critical to product efficacy. However, due to exposure to organic solvents, RP-HPLC may cause partial denaturation, limiting its use for native proteins.</p><h4><strong>Ion Exchange HPLC (IEX-HPLC)</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec"><strong>IEX-HPLC</strong></a> separates proteins based on net surface charge. Cation exchange (with negatively charged stationary phases) binds positively charged proteins, while anion exchange does the reverse.</p><p>By gradually increasing salt concentration or changing the mobile phase pH, proteins are eluted according to their isoelectric points (pI).By gradually increasing salt concentration or changing the mobile phase pH, proteins are eluted according to their isoelectric points (pI).</p><p>This approach is highly suitable for native purification, protein isoform analysis, and charge variant characterization essential steps in therapeutic protein production.</p><h4><strong>Size Exclusion Chromatography (SEC-HPLC)</strong></h4><p>SEC separates molecules based on their hydrodynamic radius. Larger proteins elute first, as they are excluded from the pores of the stationary phase, while smaller molecules penetrate and elute later.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec"><strong>SEC-HPLC</strong></a> is non-denaturing and ideal for determining protein molecular weight, oligomeric state, and aggregate content. It is frequently used as a final polishing step following ion exchange or affinity chromatography.</p><h4><strong>Affinity HPLC</strong></h4><p>Affinity-based HPLC leverages specific biological interactions between the target protein and a ligand immobilized on the stationary phase (e.g., antibodies, metal ions, or tags such as His-tag or GST).</p><p>When integrated into an HPLC system, affinity chromatography provides high selectivity and purity (&gt;95%) in a single step, often used in recombinant protein purification and biomarker capture applications.</p></div><div><div><div><div><h3><strong>Benefits of Using HPLC for Protein Purification</strong></h3><p>HPLC-based protein purification combines the analytical precision of chromatography with the preparative capability needed for research and biomanufacturing. The main advantages include:</p><h5><strong>Benefit &nbsp;/ &nbsp;Description</strong></h5><p><strong>High Resolution and Selectivity:</strong></p><ul><li>Smaller stationary phase particles and optimized flow dynamics allow HPLC to resolve proteins differing by minimal physicochemical properties. This precision is vital for distinguishing isoforms, mutants, and post-translationally modified variants.</li></ul><p><strong>Accuracy and Reproducibility</strong></p><ul><li>Automated systems maintain stable flow rates, gradient control, and detection sensitivity, ensuring reproducible results essential for regulated biopharmaceutical workflows.</li></ul><p><strong>Speed and Efficiency</strong></p><ul><li>Modern UHPLC systems equipped with fully porous or core–shell particles achieve faster separations without sacrificing resolution, reducing run times by up to 50% compared to classical methods.</li></ul><p><strong>Compatibility and Scalability</strong></p><ul><li>HPLC systems are highly adaptable, supporting both analytical-scale characterization and preparative-scale purification. Methods developed at microgram scale can be seamlessly transferred to milligram or gram scales used in process development.</li></ul><div><div><div><div><h3><strong>Factors Affecting the Efficacy of Protein Purification by HPLC</strong></h3></div></div></div></div><div><p>Optimizing HPLC for protein purification requires balancing purity, recovery, and throughput. The following parameters significantly influence results:</p><ul><li><strong>Purification goal:</strong> Define whether the priority is yield, purity, or throughput. Analytical separations prioritize resolution, while preparative runs emphasize recovery and process speed.</li><li><strong>Protein solubility and stability:</strong> Unstable or aggregation-prone proteins may require low temperatures or additives (e.g., glycerol, mild detergents).</li><li><strong>Column chemistry and particle size:</strong> Select appropriate stationary phase chemistry (silica, polymeric, or mixed-mode) and particle size to suit molecular weight and hydrophobicity.</li><li><strong>Mobile phase pH and ionic strength:</strong> pH should be optimized near but not at the protein’s pI; salt gradients influence both resolution and elution strength.</li><li><strong>Flow rate and gradient profile:</strong> Steeper gradients shorten run time but may compromise separation quality.</li><li><strong>Purified protein final form:</strong> Consider buffer composition and concentration for storage or downstream assays to maintain bioactivity.</li></ul></div><div><div><div><div><h3><strong>Best Practices for Protein Purification by HPLC</strong></h3></div></div></div></div><div><p>Following good chromatographic practices ensures consistent, high-quality results:</p><ul><li><strong>Column conditioning and storage:</strong> Always equilibrate new <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;manufacturerItems=50"><strong>HPLC columns</strong></a> with 10 column volumes of buffer; store in appropriate solvent containing preservatives.</li><li><strong>Avoid protein precipitation:</strong> Maintain appropriate ionic strength and, when native state is desired, avoid exposure to denaturing solvents or high organic content.</li><li><strong>Prevent sample loss:</strong> Use low-protein-binding tubing and vials; minimize dead volume in the system.</li><li><strong>Troubleshooting:</strong><ul><li>Peak fronting - may indicate column overloading.</li><li>Peak broadening – may indicate column channeling.</li><li>Carryover – reduce by extending wash cycles or using stronger regeneration solvents.</li><li>Clogging – prefilter samples (0.22 µm) and periodically flush the system.</li></ul></li></ul></div></div></div></div></div>

Protein Purification by HPLC: Methods, Benefits, and Best Practices

<p><strong>Scientists from CEITEC Brno University of Technology&nbsp;have succeeded in a highly competitive international selection process and secured a prestigious Human Frontier Science Program (HFSP) grant, which supports groundbreaking fundamental research. The Brno-based team led by Eric Daniel Glowacki will contribute a unique technology to an international project that enables precise control of oxygen levels in tissues. Changes in oxygen availability may play a crucial role in determining how cells specialize during early embryonic development.</strong></p><p>The project, entitled “Beyond Theory: Regulation of Mammalian Embryo Patterning by Electrochemical Gradients,” is being carried out by CEITEC BUT in collaboration with biologists from the <strong>Max Planck Institute of Molecular Cell Biology and Genetics</strong> and <strong>Yale University</strong>. The Brno team’s role is central to the project: providing technology that enables the deliberate creation of regions with different oxygen concentrations in tissues and the observation of how cells respond to these changes.</p><p><i>“Our research group specializes in electrochemistry, particularly in studying how electric current affects tissue oxygenation and the resulting chemical changes. To perform certain types of experiments, we developed our own integrated system of needle probes, electrodes, and software, which allows us to influence and control the amount of oxygen present in tissues through which current is passed,” </i>explains team leader <strong>Eric Daniel Glowacki</strong>. Using these tools, researchers can create regions within samples where oxygen is scarce or even completely absent. Changes in oxygen levels are fundamental to biology because they affect cellular metabolism and, consequently, cell behaviour. In some types of research, such precise environmental control is essential.</p><p><i>“Our system is not universal; we tailor it to each application. In the future, however, we aim to expand its variants so that it can be used across the widest possible range of research fields,”</i> adds Eric.</p><p>Scientists at the Max Planck Institute have already used CEITEC’s technology in previous studies of early embryonic development. They investigated how the metabolic activity of stem cells – undifferentiated cells capable of self-renewal and transformation into specialized cells such as muscle, nerve, or blood cells – affects both their own development and that of neighbouring cells.</p><p>The newly funded HFSP project builds on this work. Its goal is to revisit the nearly century-old theory&nbsp;proposed by the American zoologist Charles Manning Child, which explains how stem cells in multicellular organisms gradually develop into mature cells with specific functions and structures. The three-year project, supported by a grant of<strong> CZK 7.5 million </strong>for each partner, will test a new hypothesis in mouse embryos directly in the uterus: that the specific transformation of cells depends not only on oxygen depletion itself but also on the cell’s location within the tissue and the timing of the oxygen decrease.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Technologie_vedcu_z_CEITEC_pomuze_odhalit_jak_kyslik_ridi_vyvoj_embryi_2_1ea86be296.jpeg" alt="CEITEC/ Lukáš Havlák: CEITEC Technology Will Help Reveal How Oxygen Controls Embryo Development: HFSP technological setup at CEITEC BUT." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Technologie_vedcu_z_CEITEC_pomuze_odhalit_jak_kyslik_ridi_vyvoj_embryi_2_1ea86be296.jpeg 235w," sizes="100vw" width="1600" height="1067"></p><p>A better understanding of the mechanisms that govern early embryonic development could, in the future, contribute to advances in regenerative medicine, research into developmental disorders, and the more effective use of stem cells in biomedicine.</p><p><i>“The success of our project is both a great honour and a significant responsibility. HFSP is a unique programme dedicated to fundamental research, where investigators seek answers to the most basic and still unresolved questions in biology,”</i> says Eric. The programme is extremely competitive, with only two to three percent of the thousands of submitted projects receiving funding. Requirements include international collaboration across continents and a genuinely innovative approach.</p><p><i>“Through this, we have become part of a community of world-class scientists from around the globe. Although the results of this kind of research are not immediately applicable, their long-term benefit to society is immense,” </i>Eric concludes.</p><blockquote><h4><strong>About HFSP</strong></h4><p>The Human Frontier Science Program Organization (HFSPO) was established in France in 1989, although the idea of creating such an international community dedicated to fundamental research originated several years earlier in Japan. The programme funds cutting-edge research in life sciences, particularly in biology, physics, chemistry, mathematics, and computer science, and supports innovative interdisciplinary projects that would otherwise be difficult to finance.</p><p>HFSPO receives funding from governments and institutions, including those of France, Japan, Switzerland, South Korea, the United States, the European Union, Singapore, New Zealand, and the United Kingdom. Since the programme’s launch in 1989, 31 HFSP-supported scientists have gone on to receive Nobel Prizes. The most recent awardees, in 2024, were David Baker, who received the Nobel Prize in Chemistry for computational protein design, and Gary Ruvkun, who received the Nobel Prize in Physiology or Medicine for the discovery of microRNA.</p></blockquote>

CEITEC Technology Will Help Reveal How Oxygen Controls Embryo Development

<p><strong>🎤Rajas Poorna and Charles Anderson</strong></p><h4><strong>What if you could dry biofluid samples in the field—without a vacuum system, freeze dryer, or full lab setup?</strong></h4><p>In this episode of Concentrating on Chromatography, I sit down with Rajas Poorna and Charles Anderson to explore their work on a frugal, field-deployable alternative to traditional lyophilization through the Evapinator project.</p><p>Originally inspired by the principles of frugal science, their approach rethinks how we stabilize and prepare biofluid samples like blood and plasma—especially in environments where traditional lab infrastructure simply isn’t available.</p><h4><strong>What This Episode Covers:</strong></h4><h5><strong>A Frugal Take on Lyophilization</strong></h5><p>Can you replicate the outcome of a lyophilizer without the complexity?<br>Why traditional freeze drying is often too expensive and impractical for field use<br>How simplifying evaporation could unlock entirely new workflows</p><h5><strong>Drying Biofluids in the Field</strong></h5><p>The real-world challenge of handling blood and other biofluids outside the lab<br>Why sample stability during transport is such a critical bottleneck<br>How a portable system could enable on-site sample prep in remote or resource-limited settings</p><h5><strong>Rethinking Evaporation (No Vacuum Required)</strong></h5><p>The core idea behind the Evapinator—and how it challenges conventional assumptions<br>The role of airflow, heat, and design in drying sensitive samples<br>Where this approach fits relative to traditional evaporation and lyophilization techniques</p><h5><strong>Early Results &amp; Practical Insights</strong></h5><p>What’s working so far—and where limitations still exist<br>The types of applications where this approach shows the most promise<br>What still needs to be validated in upcoming peer-reviewed work</p><h4><strong>Why This Matters</strong></h4><p>From clinical research in remote regions to decentralized diagnostics, the ability to dry and preserve biofluid samples in the field could fundamentally change how and where analysis happens.</p><p>This conversation challenges a core assumption in analytical science:</p><p>Do you really need a full lyophilizer—or even a vacuum—to stabilize biofluid samples?</p><h3><strong>Video Transcription</strong></h3><p>The interview opens with a welcome to an episode of <i>Concentrating on Chromatography</i>. The host introduces guests <strong>Rajas Poorna</strong> and <strong>Charles Anderson</strong> and asks about their scientific backgrounds and how they became involved in solving evaporation challenges.</p><h4><strong>Scientific background and project origin</strong></h4><p>Rajas explains that he is trained as a physicist and is now pursuing a PhD in bioengineering. His lab focuses on building low-cost scientific equipment to make science more accessible, especially for schools and low-resource settings. This work is part of a broader “frugal science” approach, where students and researchers develop affordable versions of instruments that are normally expensive.</p><p>The original challenge came from synthetic biology education. High school teams working on synthetic biology projects needed access to equipment such as lyophilizers, but conventional systems can cost tens of thousands of dollars. The team therefore began exploring whether a more affordable lyophilizer-like system could be built for education and field use.</p><p>Charles describes joining the project early in his undergraduate studies. He was drawn to it because it had a clear practical purpose and seemed like something that could become useful outside the laboratory rather than remaining only a research prototype.</p><h4><strong>Why evaporation became the key challenge</strong></h4><p>The discussion turns to the practical limitations of traditional evaporation and drying systems. Rajas explains that during a collaboration in Thailand, researchers working on Raman spectroscopy for tuberculosis diagnostics needed to dry blood-derived samples before transporting them to a central laboratory. They used a centrifugal vacuum evaporator, but the system was slow, expensive, and difficult to service when it broke down.</p><p>This experience led to a central question: could sample drying be achieved without a vacuum? Rajas notes that everyday drying processes, such as drying clothes, do not require vacuum. This insight opened the path toward a simpler, smaller, lower-cost and potentially battery-powered drying system.</p><h4><strong>How Rajas and Charles began collaborating</strong></h4><p>Charles explains that he met the lab’s principal investigator while discussing a different project. He was then invited to work on what would later become the Evaponator. At the beginning, the project was still small and exploratory.</p><p>Rajas adds that Charles joined at a stage when the team was trying to understand the physics and engineering behind vacuum-free drying. Within a few months, they developed a prototype costing under 100 USD that could dry samples much faster than the conventional vacuum evaporator used by collaborators.</p><h4><strong>Main limitations of conventional drying systems</strong></h4><p>The interview identifies several major limitations of existing evaporation technologies:</p><ul><li>High cost</li><li>Limited reliability in remote or low-resource settings</li><li>Long drying times</li><li>Poor field portability</li><li>Challenges with sterility and cross-contamination</li></ul><p>Charles emphasizes that freeze drying can take 12–24 hours, which is impractical for field use. Rajas adds that centrifugal vacuum evaporators are not necessarily sterile and may pose a risk of cross-sample contamination. Together, these challenges shaped the requirements for the Evaponator.</p><h4><strong>What the Evaponator is</strong></h4><p>Charles introduces the Evaponator as a low-cost, portable drying system designed to dry small numbers of samples quickly without a vacuum chamber. The name was inspired by an episode of <i>Phineas and Ferb</i>.</p><p>The system is based on the same principles as drying clothes: low humidity, gentle heating and airflow. Instead of using vacuum, the Evaponator generates dry air, directs it over the sample, removes moisture through a cold trap and repeats the process in a closed or semi-closed loop.</p><p>The current design is aimed at drying approximately four 1 ml samples, which fits the intended use case of field diagnostics or low-throughput sample collection in remote settings.</p><h4><strong>How the system works</strong></h4><p>Rajas explains the operating principle in more detail. Moist air is passed through a cooled metal block using a thermoelectric cooler. As the air cools, moisture condenses inside the cold trap, producing dry air. This dry air is then directed over warmed samples, helping water evaporate while keeping the sample temperature moderate.</p><p>The system can operate in a loop to improve efficiency. HEPA filtration and aerosol filtration help prevent contamination. The team also developed an ozone-based sterilization approach, allowing the device to sterilize itself using ozone generated from air rather than relying on consumables such as ethanol or peroxide.</p><p>To prevent sample-to-sample contamination, the design uses separate airflow paths for individual samples, which merge only later in the system.</p><h4><strong>Design assumptions and engineering constraints</strong></h4><p>A key design assumption is that not every drying process needs sublimation or deep vacuum. For the biofluid applications targeted by the Evaponator, the team works around 40 °C, a temperature chosen because it is close to fever temperature and should be mild enough for many biological samples.</p><p>The engineering constraints included:</p><ul><li>Use of widely available components</li><li>No specialized machining</li><li>Bill of materials under 100 USD</li><li>Low power consumption</li><li>Portability</li><li>Simple assembly</li><li>Robust airflow without splashing or sample loss</li></ul><p>Charles notes that the team intentionally selected accessible parts, including components similar to those used in computer cooling systems, vacuum-cleaner-style HEPA filters and disposable pipette tips for directing airflow.</p><h4><strong>Sample types and solvents</strong></h4><p>The prototype is currently focused mainly on aqueous biological samples. Rajas explains that using organic solvents such as ethanol would introduce ignition risks with the current architecture, so the early work is focused on water-based samples and biofluids.</p><p>Charles adds that the system is intended primarily for blood-derived fluids, urine, saliva and similar samples where the target analytes can remain stable after drying and rehydration.</p><h4><strong>Potential analytical applications</strong></h4><p>The Evaponator is being developed for field sample preparation rather than as a replacement for every laboratory evaporator. The main idea is to dry samples near the point of collection, transport them more easily and then rehydrate them before analysis.</p><p>Potential use cases include:</p><ul><li>Field diagnostics</li><li>Biofluid sample stabilization</li><li>Remote sample collection</li><li>Clinical or public-health testing in low-resource settings</li><li>Applications where dried plasma, serum, urine or saliva can later be analyzed</li><li>Situations where conventional dried blood spot methods lack enough sample volume</li></ul><p>Rajas notes that red blood cells may not be suitable for drying directly because they can rupture, so serum or plasma is expected to be more appropriate. He also points out that oxygen exposure during forced-air drying still needs further testing for oxygen-sensitive analytes.</p><h4><strong>Current development status</strong></h4><p>Charles explains that the project is approaching important validation milestones, especially around batch-to-batch and sample-to-sample sterility. The ozone cleaning system appears promising because it may reduce the need for cleaning consumables, aside from inexpensive items such as pipette tips and occasional filter replacement.</p><p>He also mentions tests with trehalose-based solutions and collaboration with a Georgia Tech startup working on room-temperature-stable mRNA vaccines. In that context, the Evaponator was able to dry samples in about 30 minutes, compared with 12–24 hours using previous drying approaches.</p><h4><strong>Commercialization and future direction</strong></h4><p>Rajas explains that the team is exploring commercialization and has participated in a startup accelerator. They are investigating where the technology could have the strongest product-market fit, with particular interest from cost-sensitive and geographically dispersed healthcare settings, including India.</p><p>The goal is to move beyond a lab prototype toward a manufacturable, user-friendly device with a straightforward interface and reliable sealing, sterilization and operation.</p><h4><strong>Closing remarks</strong></h4><p>The interview concludes with the host thanking Rajas Poorna and Charles Anderson for sharing the technical and practical challenges behind the Evaponator. Both guests emphasize that the conversation was a valuable opportunity to explain the project in depth and reflect on its progress.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Frugal Science Meets Sample Prep: A Field-Ready Lyophilizer for Biofluids

Article

Product

Academy

Video

Scientists

Interview

Science and research

Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Tosoh Bioscience

Metrohm

Mettler-Toledo

Technology Networks

Thermo Fisher Scientific

SCIEX

Waters Corporation

<h3><strong>Solving Problems That Matter - a Waters webinar series featuring USP speakers</strong></h3>
<h3><strong>Episode 4: aQbD for OTC products</strong></h3>
<p><strong>Summary</strong></p>
<p>Analyzing over-the-counter formulations can be overwhelming due to the wide range of APIs and excipients used in varying concentrations. These excipients can cause problematic matrix effects and coelutions, making the analysis of formulations complex. Our panelists will discuss how to leverage aQbD software to develop a single chromatographic method suitable to analyze multiple APIs in a variety of formulations.</p>
<p><strong>Key learning objective:</strong></p>
<p>Learn how to leverage aQbD tools to develop a single method suitable for a variety of OTC formulations.</p>
<p><strong>Who sould attend:</strong></p>
<p>Pharmaceutical method developers </p>
<blockquote>
<p><strong>Presenter</strong>: Fadi Alkhateeb (Principle Scientist, Waters Corporations)</p>
</blockquote>
<blockquote>
<p>Dr. Fadi Alkhateeb is a Senior Scientist in the Scientific Operations Department at WatersCorporation HQ in Milford, MA USA. He joined Waters in 2017 and since then he has beenmainly focusing on exploring various approaches for analytical method development forpharmaceutical drugs. Prior to joining Waters, he was a postdoctoral fellow at the University ofCalgary (Calgary, AB, Canada), where he did research at Alberta Sulphur Research, one ofNorth America&#39;s leading Sulphur research institutes. He received his PhD from the University ofCalgary, focusing on the development of novel, rapid separation and sample preparationtechniques for the analysis of a wide range of chemical compounds. Dr. Alkhateeb publishedseveral peer-reviewed articles in top analytical journals.​</p>
</blockquote>
<blockquote>
<p><strong>Moderated by</strong>: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</p>
</blockquote>


Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

aQbD for OTC products

Solving Problems That Matter - a Waters webinar series featuring USP speakers. Episode 4: aQbD for OTC products.

<h4><strong>EU Live Interactive Web Series</strong></h4>
<p><strong>Gather on Thursday or Friday at 11 AM or 12 PM CET for our live interactive web series.</strong></p>
<p>Join us for an engaging journey into the world of advanced molecular analysis with our EU Live Interactive Web Series, hosted by Waters | Wyatt Technology! Dive deep into the intricacies of SEC-MALS and FFF-MALS systems, enhance your skills in ASTRA and DYNAMICS data processing, and master the measurement of dn/dc values—crucial for your research.</p>
<p>We dedicate 60 minutes to explore a specific topic, accompanied by a live discussion where all your questions are welcome. Whether you&#39;re dealing with polydisperse samples, eager to optimize your error analysis techniques, or curious about batch MALS work, our sessions are designed to elevate your expertise.</p>
<p>What&#39;s more, you’ll learn how to achieve stable RI baselines and utilize ASTRA&#39;s robust distribution analysis and results fitting features. Our web series is an invaluable, free resource exclusively for Wyatt customers, promising a 100% live, interactive learning experience.</p>
<p>Don’t miss out on this opportunity to connect, learn, and grow.</p>
<p>The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all Wyatt customers.</p>
<p>The EU live interactive web series are held on select Thursday or Friday afternoons at 11 AM or 12 PM CET.</p>
<p>May 3rd at 11:00 AM : MALS</p>
<ul>
<li>How MALS can characterize molar mass, radius and A2?</li>
<li>How does a DAWN detector work?</li>
</ul>
<p><strong>May 17th at 11:00 AM : SEC-MALS</strong></p>
<ul>
<li><strong>Applications and data interpretation</strong></li>
<li><strong>Data analysis in ASTRA</strong></li>
</ul>
<p>June 7th at 11:00 AM : RI &amp; dn/dc</p>
<ul>
<li>A perfect match: MALS and RI</li>
<li>The importance of dn/dc and how to determine it</li>
</ul>
<p>June 21st at 11:00 AM : DLS </p>
<ul>
<li>Benefits of measuring the Hydrodynamic Radius (Rh)</li>
<li>Dynamic Light Scattering in cuvette, high throughput and online</li>
</ul>
<p>July 5th at 11:00 AM : Dynamics data processing tips &amp; tricks</p>
<ul>
<li>Data interpretation and data filter</li>
<li>Use your Dyna Pro instrument to get: colloidal stability (kD/A2), thermal stability, viscosity or particle concentration</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Christian Ackerschott</p>
</blockquote>
<blockquote>
<p>Dr. Christian Ackerschott studied Chemistry at the University of Cologne and finished with his diploma thesis in Biochemistry. He completed his studies with the PhD thesis “Characterization of Recombinant Flagelliform Spider Silk Proteins” at the Technische Universität München, where AF4-MALS was used as an essential characterization method.</p>
</blockquote>
<blockquote>
<p>Since 2007, Christian works at Wyatt Technology as Application Scientist and is now Head of Customer Service. His tasks also include customer trainings in Dernbach and onsite as well as education and training of the local Field Application Scientists.</p>
</blockquote>


Waters Corporation: EU Live Interactive Web Series

SEC-MALS

SEC-MALS - Applications and data interpretation. Data analysis in ASTRA.

<p><strong>In this training session, the METTLER TOLEDO product managers will address the questions that are most commonly asked in the field. They will talk about weight handling, maintenance of mass comparators, selection of products, elevation dependence of measurements, and many more topics. Additionally, you will have the opportunity to ask your own questions in an open Q&amp;A session at the end.</strong></p>
<p><strong>Key Takeaways</strong></p>
<p>This is a great opportunity for all mass metrologists, as those questions that many others ask might also be of relevance to you. </p>
<p><strong>Program</strong></p>
<ul>
<li><p>Discover answers to the most frequently asked questions in mass metrology</p>
</li>
<li><p>Learn more about weight handling, mass comparator maintenance, etc.</p>
</li>
<li><p>Ask our experts your own questions</p>
</li>
</ul>
<blockquote>
<p><strong>Speaker:</strong> Dr. Frederik Eiler (METTLER TOLEDO, Product Manager Metrology Solutions)</p>
</blockquote>
<blockquote>
<p><strong>Speaker:</strong> Mark Kliebenschädel (METTLER TOLEDO, Product Manager Metrology Solutions)</p>
</blockquote>


Mettler-Toledo: Mass Calibration – Most Frequently Asked Questions

Mass Calibration – Most Frequently Asked Questions

Opportunity for all mass metrologists, as those questions that many others ask might also be of relevance to you.

<p><strong>Laboratories are constantly trying to manage their efficiency. When it comes to ion chromatography, increasing the speed of the analysis is often the first thing that comes to mind to achieve this goal. But what are the tradeoffs?</strong></p>
<p>In this webinar, we’ll take a dive into the influencing factors for chromatographic separation, what levers you have when trying to speed analysis up, what you gain and what you might give up for the compressed run time. We’ll try to help you decide where to draw the line for your lab while sharing some method examples that benefit from fast run times. Lastly, we’ll look at other strategies for efficiency like automated sample prep. </p>
<p><strong>Key Learning Points:</strong></p>
<ul>
<li>Learn about speeding up IC methods, including factors influencing chromatographic separation</li>
<li>Understand the trade-offs and things to consider when speeding up your analysis</li>
<li>Learn about alternatives to increase sample throughput when the tradeoff for speed is too much</li>
</ul>
<p><strong>Who Should Attend?</strong></p>
<ul>
<li>People developing IC methods </li>
<li>Anyone using ion chromatography</li>
<li>Lab managers and directors interested in strategies for faster lab results</li>
<li>Students and researchers looking to expand their fundamental understanding of IC</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Bikash Chouhan, PhD (Applications Specialist, Ion Chromatography, Metrohm USA)</p>
</blockquote>
<blockquote>
<p>Bikash Chouhan is an Applications Specialist for Ion Chromatography at Metrohm USA. He completed his PhD in Analytical Chemistry from the University of Texas at Arlington (UTA). His area of research during his PhD was in Capillary Scale Ion Chromatography. He has worked as a Post Doc at Department of Chemistry and Biochemistry, UTA and as a Senior Scientist in IC at Eurofins, before joining Metrohm USA. He is currently involved in expanding IC applications to more unique and challenging sample matrices.</p>
</blockquote>
<blockquote>
<p><strong>presenter:</strong> Linx Waclaski (Product Manager Ion Chromatography, Metrohm USA)</p>
</blockquote>
<blockquote>
<p>Linx Waclaski is the Product Manager for Ion Chromatography at Metrohm USA in Riverview, Florida. He has years of experience in multiple chromatographic techniques, including ion chromatography, gas chromatography, liquid chromatography, and mass spectrometry, with extensive experience in R&amp;D and applications development. His chromatography applications experience extends across environmental, food safety, and life sciences industries. He has an M.S. in Forensic Science from Duquesne University.</p>
</blockquote>


Metrohm: Speeding up Your IC: Is Faster Always Better?

Speeding up Your IC: Is Faster Always Better?

Influencing factors for chromatographic separation, levers to speed analysis up, gains and losses of the compressed run time.

<p><strong>One of the key aspects of water quality assurance is monitoring disinfection byproducts (DBPs), especially haloacetic acids (HAAs), bromate, and dalapon.</strong></p>
<p><strong>As these substances pose a significant health risk, regulatory bodies across the world, such as the US EPA and the European Union, have established strict guidelines to limit their presence in drinking water. The EPA Method 557 by the US EPA standardizes the comprehensive monitoring of 9 HAAs, bromate, and dalapon.</strong></p>
<p>This webinar will discuss the latest advancements in analytical techniques and instrumentation that meet the regulatory requirements. The focus will be on exploring the capabilities of ion chromatography coupled with mass spectrometry (IC-MS and IC-MS/MS), a method that is highly sensitive, reliable, and efficient in detecting low levels of HAAs and other DBPs without requiring extensive sample preparation.</p>
<p>Attendees will have the opportunity to witness the seamless integration of HAA analysis by IC-ESI-MS/MS and IC-ESI-MS within water laboratories, demonstrating consistent results and efficient lab processes. Our experts will showcase how ion chromatography and mass spectrometry provide a streamlined workflow, from sample injection to analysis, significantly reducing manual labor and associated costs. </p>
<h5><strong>Key learnings of this webinar:</strong></h5>
<ul>
<li>Learn how IC coupled to mass spectrometry is the ideal and most reliable technique for analysis of HAA, bromate, and dalapon in drinking water</li>
<li>Develop an understanding of how ion chromatography and mass spectrometry can complement each other to enhance selectivity and sensitivity</li>
<li>Learn to know Metrohm and Agilent as your reliable lab partners</li>
</ul>
<h5><strong>Who should attend?</strong></h5>
<ul>
<li>Water utility operators who need robust and dependable analytical techniques to guarantee the safety of drinking water</li>
<li>Scientists, researchers, and analytical method developers who need to stay up-to date on state-of-the-art methods for water analysis</li>
<li>Laboratory staff and managers considering to implement reliable, cost- and time-efficient analysis of HAA in their lab</li>
</ul>


Metrohm: Water safety: Improve your analysis of haloacetic acids with IC-ESI-MS/MS

Water safety: Improve your analysis of haloacetic acids with IC-ESI-MS/MS

The latest advancements in analytical techniques that meet the regulatory requirements in monitoring disinfection byproducts (DBPs).

Improve your analysis of haloacetic acids with IC-ESI-MS/MS

<h5><strong>Overview:</strong></h5>
<ul>
<li>The reporting limit of a method is also dependent upon the consistency of clean blanks and elimination of sporadic contamination.</li>
<li>Vendors of PFAS consumables need to provide certified, trace level PFAS free products.</li>
<li>PFAS are everywhere!  They are widely used, vendors/suppliers when contacted don&#39;t know their processes are using them.</li>
<li>Since PFAS contamination has become an issue with PFAS analysis all field samples are taken in duplicate, not just field blanks. </li>
<li>The complete data package and replicates need to be evaluated by the user in order to make sound conclusions and identify false positives. </li>
<li>Every step there is a chance to incorporate “free-range” PFAS in your sample, minimize the preparation steps</li>
<li>How PFAS is analyzed at EPA Region 5 Laboratory</li>
</ul>
<h5><strong>Who Should Attend:</strong></h5>
<ul>
<li>Anyone lab scientists, or managers involved in PFAS Analysis</li>
</ul>
<h5><strong>Key Learning Points:</strong></h5>
<ul>
<li>Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be</li>
<li>Become aware of Sporadic PFAS Contamination</li>
<li>Understand how to identify false positives</li>
<li>Require all analysis quality control and samples to be analyzed in replicates to identify false positives.</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Larry Zintek (Organic Methods Group Leader, UA EPA)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Maryam Sayeed (Product Management Specialist, Phenomenex)</p>
</blockquote>


Phenomenex: Identifying and Avoid False Positives During Routine PFAS Analysis

Identifying and Avoid False Positives During Routine PFAS Analysis

Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be.

False Positives During Routine PFAS Analysis

<h4><strong>LC-MS/MS fundamentals webinar series 2.0</strong></h4>
<p><strong>Advanced techniques for data acquisition and processing</strong></p>
<p><strong>Elevate your analytical expertise with advanced techniques and workflows</strong></p>
<p>Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series. Based on our LC-MS/MS 1.0 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows.</p>
<p>Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.</p>
<h4><strong>This webinar:</strong></h4>
<p>May 21, 2024 11:00 a.m. - 11:30 a.m. CET - <strong>Advanced MS/MS method building</strong> </p>
<p>Gain insight into advanced features for maximizing sensitivity in your mass spectrometry analyses.</p>
<h4><strong>Other webinars from the series:</strong></h4>
<p>May 7, 2024 11:00 a.m. - 11:30 a.m. CET    - <a href="https://lcms.labrulez.com/webinar/3820"><strong>Beyond the fundamentals</strong></a></p>
<p>June 4, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3853"><strong>Mastering chromatography</strong></a></p>
<p>Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.</p>
<p>June 18, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3854"><strong>It&#39;s a TRAP! Getting the most out of your QTRAP system</strong></a></p>
<p>Learn when to utilize QTRAP functionality to create more selective and sensitive methods.<br/>July 2, 2024</p>
<p>11:00 a.m. - 11:30 a.m. CET    - <a href="https://lcms.labrulez.com/webinar/3855"><strong>Troubleshooting at the source</strong></a></p>
<p>Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.</p>
<p>July 16, 2024 11:00 a.m. - 11:30 a.m. CET    <a href="https://lcms.labrulez.com/webinar/3856"><strong>Automating data processing</strong></a></p>
<p>Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.</p>
<p>July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3857"><strong>Excel strategies for mass spectrometry data analysis</strong></a></p>
<p>Take an in-depth look at when and how to use Excel to increase your productivity.</p>


SCIEX: LC-MS/MS fundamentals webinar series 2.0

Advanced MS/MS method building

Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series.

<p><strong>The new Thermo Scientific Orbitrap Astral mass spectrometer is setting a new standard for mass spectrometry performance, redefining what is possible and unlocking additional insights into biology and disease mechanisms.</strong></p>
<p>The ability to conduct large cohort protein analysis with faster throughput, deeper coverage, higher sensitivity and accurate and precise quantitation enables a better understanding of the dynamic, temporal and spatial complexities of biology.</p>
<p>In this interactive webinar, we will briefly introduce underlying technology of the Orbitrap Astral mass spectrometry platform before opening the floor to a group of renowned cancer researchers who will describe their own early experiences with the system and their hopes and ambitions for advancing their own researches in this important and rapidly-advancing field.</p>
<p>As a participants in this webinar, you will have the opportunity to engage in an interactive panel discussion with these key opinion leaders and ask questions to further enhance your understanding of the current and future state of mass spectrometry-driven proteome analysis of cancer samples and how that can identify differentially expressed proteins, protein-protein interactions and post-translational modifications that may be associated with cancer development, progression, or response to treatment.</p>
<p><strong>What you will learn:</strong></p>
<ul>
<li>View the latest research in protein profiling</li>
<li>Learn about the Orbitrap mass spectrometry solutions</li>
<li>Discover how your cancer research can be empowered</li>
</ul>
<p>Who should attend?</p>
<ul>
<li>Scientific researchers in the field of cancer proteomics</li>
<li>Mass spectrometrists working in core labs in cancer institutes</li>
<li>Laboratory managers in cancer institutes</li>
</ul>
<p>If you have questions for our experts, please submit them during registrations or send them anytime in advance via email to analyze.eu@thermofisher.com</p>
<p>Live questions will be handled during the webinar in our Q&amp;A chat. </p>
<p>Short notice: Can&#39;t attend the live broadcast? No problem! Register anyway and you will automatically receive the link to the webinar recording.</p>
<blockquote>
<p><strong>Presenter:</strong> Dr. Christian Hock (Senior Manager Instrument Development, Thermo Fisher Scientific)</p>
</blockquote>
<blockquote>
<p>The Orbitrap Astral development team is led by Dr. Christian Hock, who joined the Research team of Dr. Alexander Makarov as Lead Scientist in early 2013. With a strong passion for science and an innate curiosity, Dr. Hock has been involved in all aspects of Astral technology development, from its initial stages of basic research to its successful transition into serial production.</p>
</blockquote>
<blockquote>
<p>In 2017, Dr. Hock transferred to the Bremen R&amp;D organization, where he took the initiative to establish a dedicated R&amp;D team which has been instrumental in bringing the Orbitrap Astral product to fruition. His overarching goal is to exceed customer expectations by delivering cutting-edge products that enable researchers worldwide to accelerate their scientific output. Through his leadership and expertise, he continuously pushes the boundaries of science &amp; technology and strives for excellence in every stage of product development.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Prof. Michiel Vermeulen (Senior Group Leader, Netherlands Cancer Institure NKI)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Harmjan Vos (Head of the Proteomics Facility, University Medical Centre Utrecht)</p>
</blockquote>
<blockquote>
<p>Started his proteomics career as a postdoc in a project of Jeroen Krijgsveld in the lab of Albert Heck at the UU. Then he moved to the lab of Boudewijn Burgering at the Center for Molecular Medicine (CMM) at the UMC Utrecht, trying to understand the intricate connections between cellular signaling pathways and metabolic processes. Since 2014 he is heading the Proteomics facility at CMM, which is involved in the research infrastructure of the Oncode Institute, with an emphasis on single cell proteomics.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Prof. Jyoti Choudhary (Group Leader, The Institute of Cancer Research)</p>
</blockquote>
<blockquote>
<p>Professor Jyoti Choudhary&#39;s research focuses on the development and implementation of novel mass spectrometry and data analysis approaches for proteome discovery. She is Head of the Proteomics Core Facility and Career Faculty Leader of the Functional Proteomics Research Group.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Joanna Kirkpatrick (Technical Sales Specialist, Proteomics, Thermo Fisher Scientific)</p>
</blockquote>
<blockquote>
<p>Jo has a degree in Chemistry from the University of York and a PhD in analytical chemistry from the University of Reading. Following a post-doc in mass spectrometry in the Chemistry department at Oxford, and working as an application scientist at Waters, she has worked in and ran Proteomics Core Facilities in the UK and Germany, including the EMBL in Heidelberg, the Leibniz Institute on Aging in Jena, The Francis Crick Institute in London and CRUK Cambridge.</p>
</blockquote>
<blockquote>
<p>After many years as an experienced Thermo customer, Jo joined the company last year as a Technical Sales Specialist, supporting the whole of the UK/Ireland, Benelux and Nordic regions, in all things proteomics.</p>
</blockquote>
<blockquote>
<p>When she&#39;s not talking about proteomics and mass spectrometry, Jo enjoys ceroc dancing, tennis, scuba diving, travelling, and spending time with her cats, who like to join in on Teams meetings whenever she works from home.</p>
</blockquote>


ThermoFisher Scientific: High throughput protein profiling for cancer research by Orbitrap Astral MS

High throughput protein profiling for cancer research by Orbitrap Astral MS

Cancer research by Orbitrap Astral MS

<p><strong>New for 2024, this online event will distill complex scientific concepts into concise, informative 10-minute presentations. Our expert panel of speakers will cover sources, impacts and mitigation strategies for environmental pollutants, providing a comprehensive overview of the challenges and solutions in this highly topical field.</strong></p>
<h5>This online event will explore:</h5>
<ul>
<li><strong>The impact and emission of particles from road vehicles</strong></li>
<li><strong>Global concerns and persistence of poly- and perfluorinated alkyl substances</strong></li>
<li><strong>Sample preparation for the analysis of environmental pollutants</strong></li>
<li><strong>The environmental impacts of synthetic chemicals</strong></li>
<li><strong>Environmental pollutants and media in our everyday space</strong></li>
</ul>
<h5><strong>17:00 – 17:15 The Environmental Impacts of Synthetic Chemicals</strong></h5>
<ul>
<li>Alistair Boxall, PhD (University of York)</li>
</ul>
<h5><strong>17:10 – 17:25 Talk Title to be Confirmed</strong></h5>
<h5><strong>17:25 – 17:35 The Problem of PFAS Here, PFAS There, PFAS Everywhere</strong></h5>
<ul>
<li>Rainer Lohman, PhD (University of Rhode Island)</li>
</ul>
<h5><strong>17:35 – 17:45 Creating the Right Environment for Indoor Air Quality</strong></h5>
<ul>
<li>Greg Davies (Assurity Consulting)</li>
</ul>
<h5><strong>17:45 – 17:55 Environmental Pollutants and Media in Our Everyday Space</strong></h5>
<ul>
<li>Jamie Mitri (Moss Pure)</li>
</ul>
<h5><strong>17:55 – 18:05 Are Electric Vehicles Zero Emissions?</strong></h5>
<ul>
<li>David Green, PhD (Imperial College London)</li>
</ul>
<h5><strong>18:05 – 18:15 Sample Preparation for the Analysis of Environmental Pollutants</strong></h5>
<ul>
<li>Peter Baugh, PhD (The BMSS)</li>
</ul>
<h5><strong>18:15 Event Close</strong></h5>
<blockquote>
<p><strong>Presenter:</strong> Greg Davies (Director of Market Development, Assurity Consulting)</p>
</blockquote>
<blockquote>
<p>Greg has worked for over 35 years in health, safety and environmental compliance management covering aspects including Legionella, H&amp;S, fire, asbestos, building environment quality, energy, waste and sustainability. A degree-qualified microbiologist and registered expert witness, he joined Assurity Consulting in 1989. Greg is a Fellow of the Royal Society of Public Health, Specialist Member of the International Institute of Risk and Safety Management (IIRSM), is a committee member of the IWFM Sustainability Special Interest Group and Chair’s the IIRSM London Branch committee. Greg is a regular blogger and contributor to our industry press, with over 250 articles published on environmental management/sustainability, health and safety and risk. He chaired the CIBSE committee that revised TM13, contributed to CIBSE Guides A and M, was the author of the original IWFM good practise guide to risk management and has been survey lead for the IWFM sustainability in FM survey. On subjects ranging from water management to the “Red Tape Challenge” Greg has also spoken at events for, or been involved with, organisations including Defra, PHE, IIRSM, DECC, BSC, RICS, CIEH, BSRIA, CIBSE, IWFM, IOSH, CIEH and the London Health and Safety Group.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Jamie Mitri (Founder and CEO, Moss Pure)</p>
</blockquote>
<blockquote>
<p>Jamie Mitri is an Entrepreneur with degrees in Chemical Engineering and Biology. Jamie spent her career developing and manufacturing medical devices and biotechnology products used in the ICU of hospitals, overseeing environmental compliance, including air pollution and wastewater, and more recently as a project manager focusing on business strategy and marketing. Jamie founded her company, Moss Pure, at a startup competition at the Massachusetts Institute of Technology, where it won First Place Startup (MIT Lebanon Challenge). Jamie then participated in the United Nations Development Programme, where Moss Pure won Top 10 Startup. Most moss companies use preserved or dried moss which is no longer living. Moss Pure is the world&#39;s only live moss air filter and stress relief device, while acting as an aesthetically pleasing decor piece in your home and office. No watering, sunlight, or maintenance is needed as Moss Pure&#39;s proprietary engineering sustains the live moss with the product itself, working in synergy to allow moss to obtain its nutrients from the air and become an air filter. Jamie uses both her engineering and science skills in the upstream process of the assembly of each piece, where the multi-layered air filter is built, and then uses her love and skill in fashion and interior design in the downstream process to produce a unique, aesthetically pleasing art piece for your space. For more information, feel free to contact Jamie and visit www.mosspure.com.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Peter J Baugh, BSc, PhD, FRSC (Co-leader of the Environmental and Food Analysis Special Interest, The BMSS)</p>
</blockquote>
<blockquote>
<p>BSc (Hons., 1961) and PhD (1964), University of Wales, retired from the University of Salford, Department of Chemistry and Applied Chemistry, 1967 – 2000. Currently, co-leader of the environmental and food analysis special interest of the BMSS, past visiting professor at the University of Chester, life member of the BMSS for services to mass spectrometry.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Aaron Hineman (Inorganic Product Line Leader, PerkinElmer)</p>
</blockquote>
<blockquote>
<p>Aaron Hineman is Americas Inorganic Product Line Leader at PerkinElmer. He is a skilled speaker and trainer with over 20 years of expertise in inorganic analytical chemistry. He spent a decade developing analytical methodologies for international operations. Aaron&#39;s extensive knowledge spans the implementation of atomic spectroscopic technologies, including ICP-MS and ICP-OES instrumentation, as well as hyphenated speciation techniques. </p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Rainer Lohmann, PhD (Professor of Oceanography, Director of the URI Superfund Center on PFAS, University of Rhode Island)</p>
</blockquote>
<blockquote>
<p>Dr. Rainer Lohmann is a Professor at the University of Rhode Island’s Graduate School of Oceanography. He obtained a degree in Chemical Engineering and a Doctorate in Environmental Science. He is Director of the URI-led Superfund Research Program Center on the Sources, Transport, Exposure and Effects of PFAS (STEEP). His research focuses on the detection, bioaccumulation, transport and fate of anthropogenic pollutants in the environment, often relying on passive samplers in the process. Since 2000, he has published &gt; 180 publications and book chapters. Dr. Lohmann was member of the U.S. EPA’s Board of Scientific Counselors (2017-2023) and serves as Editor of Environmental Toxicology and Chemistry. He was awarded a Fulbright Fellowship as part of the Fulbright Arctic Initiative III in 2020, and an Alexander-von-Humboldt Fellowship in 2011.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> David Green, PhD (Senior Research Fellow, Imperial College London)</p>
</blockquote>
<blockquote>
<p>Dr David Green is a Senior Research Fellow in the Environmental Research Group where he leads the Aerosol Science Team. Research interests focus on the measurement of particles and gases, and their use in source apportionment and health studies, to improve our understanding in both the urban and global environment. Source apportionment methodologies are used to interpret the high time resolution chemical composition measurements and quantify the contribution of different emission sources in complex urban environments. In these environments a wide variety of sources, both local (such as traffic) and distant (such as that from agricultural sources), contribute to the total particle load in the atmosphere.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Alistair Boxall, PhD (Alistair Boxall, PhD, Professor of Environmental Science, University of York)</p>
</blockquote>
<blockquote>
<p>Alistair Boxall is Professor in Environmental Science in the Environment Department and Director of the NERC-funded ECORISC Centre for Doctoral Training. Alistair’s research focuses on understanding emerging and future ecological and health risks posed by chemical contaminants in the natural environment.</p>
</blockquote>


Technology Networks: Teach Me in 10: Environmental Pollutants

Teach Me in 10: Environmental Pollutants

Sources, impacts and mitigation strategies for environmental pollutants, providing a comprehensive overview of the challenges and solutions.

<p><strong>Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.</strong></p>
<p>Dr. Carsten Arlt, Application Scientist at Tosoh Bioscience and Patrick Endres, Manager - Product Management EMEA, will explain the advantages of a novel Protein A chromatography resin for the purification of antibodies in batch and continuous applications. They will give an overview of the requirements and advancements in Protein A chromatography, show how a mAb purification process can be transferred from batch to continuous chromatography and what advantages this offers.</p>
<p>After the webinar, ask your questions during the live Q&amp;A session.</p>
<blockquote>
<p><strong>Presenter:</strong> Christian Rohrer (Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH)</p>
</blockquote>


Tosoh Bioscience: Tosoh Tuesday - Advances in mAb Purification from Batch to Continuous

Advances in mAb Purification from Batch to Continuous

The advantages of a novel Protein A chromatography resin for the purification of antibodies in batch and continuous applications.