News from LabRulezLCMS Library - Week 15, 2026

<p><strong>Traditional protein purification workflows in many laboratories still rely on ultracentrifugation and long incubation steps. These approaches often involve multi-step protocols, extended processing times, and the use of specialized equipment. As a result, workflows can become complex, time-consuming, and prone to variability.</strong></p><p>Magnetic bead-based protein purification offers a faster, simpler, and more efficient alternative by enabling selective capture and rapid magnetic separation.</p><h3><strong>Limitations of Conventional Protein Cleanup Methods</strong></h3><p><strong>In standard laboratory workflows, protein cleanup typically involves:</strong></p><ul><li>Multiple processing steps</li><li>Centrifugation and column equilibration</li><li>Dependence on specialized instrumentation</li><li>Long processing times</li></ul><p><strong>These factors introduce several challenges:</strong></p><ul><li>Increased complexity of workflows</li><li>Variability between runs, reducing reproducibility</li><li>Risk of sample loss during transfers, especially for small sample volumes or scarce targets</li><li>Limited throughput due to manual handling</li></ul><h3><strong>Principle of Magnetic Bead-Based Purification</strong></h3><p>Magnetic beads enable selective protein capture through surface functionalization.</p><p>Each bead is coated with <strong>streptavidin</strong>, allowing the attachment of a <strong>biotinylated ligand</strong>. This ligand can be selected based on the target analyte and may include:</p><ul><li>Antibodies</li><li>Aptamers</li><li>Antigen fragments</li></ul><p>This functionalization step provides the beads with the specificity required to bind the target analyte.</p><h3><strong>Materials and Key Components</strong></h3><h4><strong>Magnetic Beads</strong></h4><ul><li>Streptavidin-coated surface</li><li>Functionalized with biotinylated ligands</li></ul><h3><strong>Ligands</strong></h3><ul><li>Selected for target specificity</li><li>Attached to beads prior to purification</li></ul><h3><strong>Step-by-Step Procedure</strong></h3><h4><strong>1. Bead Activation</strong></h4><p>Load the biotinylated ligand onto the streptavidin-coated magnetic beads to confer specificity.</p><h4><strong>2. Washing</strong></h4><p>Remove impurities after ligand loading to prepare the beads for interaction with the sample.</p><h4><strong>3. Incubation with Sample</strong></h4><p>Incubate the functionalized beads with the sample to allow binding between the ligand and the target analyte.</p><h4><strong>4. Magnetic Separation</strong></h4><p>Place the sample on a magnet:</p><ul><li>Magnetic beads (with bound analyte) are attracted to the vessel wall</li><li>The liquid phase can be separated easily</li></ul><h4><strong>5. Washing</strong></h4><p>Wash the beads while they remain immobilized by the magnetic field to remove:</p><ul><li>Weak interactions</li><li>Non-specific binding</li></ul><h4><strong>6. Elution</strong></h4><p>Add an elution solvent to release the purified analyte from the beads.</p><h3><strong>Downstream Applications</strong></h3><p>After elution, the purified sample is ready for further analysis, including:</p><ul><li>LC-MS</li><li>Western blot</li><li>ELISA</li><li>Activity assays</li></ul><h4><strong>Advantages of Magnetic Bead-Based Workflows</strong></h4><p>Magnetic bead-based purification offers several key benefits:</p><h4><strong>Speed and Simplicity</strong></h4><ul><li>No centrifugation required</li><li>No complex instrumentation needed</li></ul><h4><strong>Improved Reproducibility</strong></h4><ul><li>Consistent magnetic separation</li><li>Reduced variability between runs</li></ul><h4><strong>Reduced Sample Loss</strong></h4><ul><li>Fewer transfer steps</li><li>Suitable for small or scarce samples</li></ul><h4><strong>Scalability and Throughput</strong></h4><ul><li>Compatible with automation</li><li>Supports microplate formats:<ul><li>96-well plates</li><li>384-well plates</li></ul></li></ul><h3><strong>Biozen™ Magnetic Beads Features</strong></h3><p>Phenomenex <strong>Biozen™ magnetic beads</strong> incorporate several design features:</p><ul><li><strong>Hydrophilic co-polymer surface</strong><ul><li>Reduces non-specific hydrophobic interactions</li></ul></li><li><strong>Biotinylated BSA spacer</strong><ul><li>Acts as a linker to streptavidin</li><li>Helps orient capture ligands</li></ul></li><li><strong>Monodispersed capture ligands</strong><ul><li>Optimized binding capacity</li></ul></li></ul><p>These features contribute to:</p><ul><li>Reduced non-specific binding</li><li>Improved capture consistency</li><li>Cleaner eluates</li></ul><h3><strong>When to Use Magnetic Beads</strong></h3><p>Magnetic bead-based purification is particularly suitable when:</p><ul><li>Sample volumes are small</li><li>Fast turnaround is required</li><li>Minimal equipment is preferred</li><li>High-throughput workflows are needed</li></ul><h3><strong>Conclusion</strong></h3><p>Magnetic bead-based protein purification simplifies traditional workflows by eliminating centrifugation and reducing complexity. Through selective capture and efficient magnetic separation, it enables faster processing, improved reproducibility, and compatibility with high-throughput and automated systems.</p>

Magnetic Bead-Based Protein Purification: A Faster and More Efficient Workflow

Microplastics and Nanomaterials: Understanding Emerging Contaminants in Environmental Testing

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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<p><strong>Untargeted metabolomics provides an opportunity to interrogate a sample for systems biology and biomarker discovery for life science researchers.</strong></p>
<p>Discussed here is a comprehensive untargeted analysis workflow for metabolomics.</p>
<p>Plasma samples from mouse models were processed using automated sample prep workflow and analyzed using HILIC chromatography and the 6546 LC/Q-TOF which provided high resolution, extended dynamic range, and isotopic fidelity for the highest confidence in identification. A custom and transferable MS/MS and retention time library for over 400 core metabolites was built for identification. When a true unknown was discovered, SIRIUS/CSI:FingerID software was used for identification based on the MS/MS fragment profile.</p>
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<p><strong>Presenter: Karen Yannell (LC/MS Application Engineer, Agilent Technologies, Inc.)</strong></p>
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<p>Karen E. Yannell is a LC/MS Application Engineer at Agilent Technologies. She is an expert in small molecule high throughput analysis by triple quadrupole (TQ) and quadrupole time-of-flight (QTOF) mass spectrometry for applied markets. Her recent work has focused on developing routine Q-TOF workflows for both expert and non-expert users alike. Previously, Karen worked on forensic, metabolomics, and biomarker discovery applications with ambient ionization sources. She received her PhD in Analytical Chemistry from Purdue University where studied under the advisement of Prof. R. Graham Cooks. Outside of the laboratory, she enjoys cooking unique dishes and hiking along the beach with her dog.</p>
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Agilent Technologies: Say “I Do” to New ID Tools: A Novel Untargeted Approach to Unveil the Metabolome

Say “I Do” to New ID Tools: A Novel Untargeted Approach to Unveil the Metabolome

Discussed here is a comprehensive untargeted analysis workflow for metabolomics.

A Novel Untargeted Approach to Unveil the Metabolome

<p><strong>Measurements of free hormone levels are routinely performed in clinical applications to help diagnose health concerns. There are two primary methods that have been used for quantification: immunoassays and liquid chromatography tandem mass spectrometry (LC-MS/MS) usually in conjunction with a sample preparatory technique like equilibrium dialysis (ED); with ED considered the “gold-standard.”</strong></p>
<p>A new technology, BioSPME (Bioanalytical Solid Phase MicroExtraction), has been developed to provide a high throughput automatable cleaner sample in a fraction of time compared to ED.</p>
<h5><strong>In this webinar, we will discuss:</strong></h5>
<ul>
<li>What is BioSPME</li>
<li>How does BioSPME work</li>
<li>Several applications of clinically relevant free hormones correlated against validated equilibrium dialysis samples</li>
</ul>
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<p><strong>Presenter:</strong> M. James Ross (Senior Scientist)</p>
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<p>M. James Ross is a senior scientist at MilliporeSigma. In his past 2.5 years with the company, he has focused on sample preparation primarily dealing with biological samples. James received his BA in Chemistry from Hiram College and his Ph.D. from The Ohio State University.</p>
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MERCK: BioSPME: A Novel Sample Preparation of Clinically Relevant Analytes

BioSPME: A Novel Sample Preparation of Clinically Relevant Analytes

A new technology, BioSPME has been developed to provide a high throughput automatable cleaner sample in a fraction of time compared to ED.

BioSPME: A Sample Prep of Clinically Relevant Analytes

<p><strong>Peptide fractionation is important in proteomics workflows to reduce sample complexity and increase the separation efficiency. This leads to increased dynamic range of the identified peptides compared to conventional single-shot analysis.</strong></p>
<p>The additional number of injections per fractionated sample is a good match with the short overhead time between injections on the Evosep One.</p>
<h4><strong>Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing</strong></h4>
<ul>
<li><strong>Presenter:</strong> Claire Koenig (CPR)</li>
</ul>
<p>Combination of isobaric labeling (TMTs) coupled with offline high-pH fractionation can maximize analysis depth in quantitative phosphoproteomics analysis. To investigate to what extent sample amount affect the sensitivity and the dynamic range of the analysis, we benchmark strategies for quantitative phosphoproteomics with limited peptide input amounts. Based on this research, we present a decision tree to guide phosphoproteomics users to find the best workflow, to reach optimal depth of coverage, based on experimental design and peptide availability.</p>
<p><strong>More to be announced</strong></p>


Fractionation

Peptide fractionation is important in proteomics workflows to reduce sample complexity and increase the separation efficiency. 

<p><strong>You have seen a fluorescence signal from your sample, but is it the best you can do? This Workshop will focus on the basics of the Cary Eclipse fluorescence software and its operation as well as the optimization of the observed signal. The relationships of spectral bandwidth and detector voltage on the observed signal will be examined. Additional topics to be discussed include the inner filter effect and its manifestation as well as the elimination of second order Rayleigh Scatter.</strong></p>
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<p><strong>Presenter:</strong> Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)</p>
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<p>Mark, an Application Scientist for Agilent Technologies, has over 30 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.</p>
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Agilent Technologies: Optimization of Fluorescence Signals

Optimization of Fluorescence Signals

This Workshop will focus on the basics of the Cary Eclipse fluorescence software and its operation as well as the optimization of the observed signal.

<p><strong>In this educational webinar series, we will go back to the basics of  chromatography, mass spectrometry, data analysis and troubleshooting. Understanding the importance of the basics will enable you to be more effective in your laboratory. During each session we will cover a specific aspect of your LC or LC/MS system:</strong></p>
<ul>
<li>The theory of chromatography and requirements for great separations</li>
<li>The basics of your Liquid Chromatograph (LC): Pumps, injectors, and detectors</li>
<li>An Introduction to mass spectrometry (MS) systems</li>
<li>Considerations with System Suitability</li>
<li>Troubleshooting your LC and MS systems</li>
</ul>
<h3><strong>PART 5  |  SERVICE</strong></h3>
<ul>
<li><strong>Troubleshooting your LC and MS Systems in your Laboratory</strong></li>
<li><strong>When to Start Thinking about Instrument Replacement</strong></li>
</ul>
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<p><strong>Presenter:</strong> Sharon Munisamy, Ph.D. (Principal Systems Specialist, Waters Corporation)</p>
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<p>Sharon Munisamy is a Principal Systems Specialist, based in New England. She specializes in ACQUITY™ UPLC and Time-of-Flight MS systems, providing support to customers as well as mentoring and training new engineers on these products. Prior to joining Waters, Sharon developed LC/MS and ion mobility methods for the study of biomass byproducts and asphaltenes at Baylor University.</p>
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<p><strong>HOST:</strong> Alexandra (Allie) Ahern (Informatics Product Marketing Manager, Americas Field Marketing, Waters Corporation)</p>
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<p>Allie Ahern has been with Waters for 10 years and is the Informatics Product Marketing Manager within the Americas Field Marketing Organization. Prior to Allie’s current role, she has held other roles at Waters including Service Marketing Product Manager, Implementation Project Manager, and Field Service Engineer.</p>
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<p><strong>HOST:</strong> Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)</p>
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<p>Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.</p>
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Waters Corporation: Fall Back to Basics - Part 5: Service

Fall Back to Basics - Part 5: Service

PART 5 topics: Troubleshooting your LC and MS Systems in your Laboratory. When to Start Thinking about Instrument Replacement.

<p><strong>This webinar will discuss considerations for adaptation and optimization of an AAA pre-column derivatization method from UPLC to HPLC/UHPLC. The following topics will be examined:</strong></p>
<ul>
<li>Shorter run times (i.e., higher throughput and lower solvent consumption) using a new column for AAA vs. traditional HPLC methods</li>
<li>How to accurately separate, identify, &amp; quantify amino acids in cell culture, protein hydrolysates, foods, and feeds </li>
<li>Scaling of AccQ·Tag Ultra UPLC methods for analysis on HPLC systems, including Waters Arc HPLC and ACQUITY Arc Chromatography Systems</li>
</ul>
<h5>Key learning objectives:</h5>
<ul>
<li><strong>How to accurately separate, identify, &amp; quantify amino acids in cell culture, protein hydrolysates, foods, and feeds</strong></li>
<li><strong>Scaling of proven AccQ·Tag Ultra UPLC methods for analysis on HPLC systems, including Waters Arc HPLC and ACQUITY Arc Chromatography Systems</strong></li>
</ul>
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<p><strong>Presenter:</strong> Paula Phenix (Principal Product Marketing Manager, Waters Corporation)</p>
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<p>Paula Phenix is a Principal Product Marketing Manager in the Chemistry and Automation Group at Waters Corporation, where she has been for three years. Prior to the Chemistry and Automation Group, she was a Principal Product Marketing Manager in the LC Instruments Product Management team.</p>
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<p><strong>Presenter:</strong> Kimberly Martin (Evaluation Scientist II, Waters Corporation)</p>
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<p>Kimberly Martin is part of the System Design and Development Team at Waters Corporation and serves as an Evaluation Scientist II that verifies, validates and tests new instrumentation and products. She has been the company for over 4 years. She has a total of 13 years of analytical chemistry experience including polymer chemistry, food and environmental chemistry, and pharmaceutical chemistry. Kimberly has extensive experience in HPLC, LCMS, GCMS, GC-FID, additional MS technologies and sample preparation.</p>
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Waters Corporation: Updating your HPLC Method for Amino Acid Analysis to Save Time Compared to Traditional HPLC

Updating your HPLC Method for Amino Acid Analysis to Save Time Compared to Traditional HPLC

This webinar will discuss considerations for adaptation and optimization of an AAA pre-column derivatization method from UPLC to HPLC/UHPLC.

Updating your HPLC Method for Amino Acid Analysis

<p><strong>According to the PIC/S guidance complex or open-ended processes that lead to subjective interpretation, can result in an increase in data failures.  One way to reduce this risk is with a simplified, well-defined process.  Empower software enables you with tools to create structure in your chromatographic processes and workflow.  Leveraging a well-defined workflow along with a clear SOP that details important steps, such as how to work thru reprocessing of data, will reduce the risk of failures.  More complexity added to a workflow can result in an increase in data review requirements and subsequently the associated audit trails.</strong></p>
<p>In this workshop we will define a chromatographic workflow with Empower software to support data integrity goals.  Starting with method development, method validation, and routine usage to data acquisition, data processing, reporting results, and using control charts to track and trend data.  </p>
<p>Please join us for this live discussion to learn more and bring your Empower software questions for this exclusive opportunity to ask our expert, Neil Lander, anything about Empower CDS!</p>
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<p><strong>Presenter:</strong> Neil Lander (Principal Product Manager, Waters Corporation)</p>
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<p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p>
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Waters Corporation: Empower Tips: Ask Me Anything About Creating Efficiency in Your Routine Workflow with Simple Automation Steps

Empower Tips: Ask Me Anything About Creating Efficiency in Your Routine Workflow with Simple Automation Steps

In this workshop we will define a chromatographic workflow with Empower software to support data integrity goals.

Empower Tips: Simple Automation Steps

<p><strong>Monitoring the quality of hydraulic and/or lube oils is critical to prevent premature wear of any kind of machinery. In this session we will look at this topic from a new perspective focusing on the combination of titration (potentiometric, thermometric, coulometric Karl Fischer titration) and Vis-Near-Infrared Spectroscopy (Vis-NIRS).</strong></p>
<p>Combining titration (highly sensitive, specific, a widely used primary method) with Vis-NIRS (fast, non-destructive, results for multiple parameters at once) can make your oil condition monitoring and/or lubricant analysis much more efficient, especially, if your laboratory has to analyze large sample series every day.</p>
<p>Attending this session, you will learn and understand:</p>
<ul>
<li>The specific advantages of each method (titration and Vis-NIR-Spectroscopy)</li>
<li>How and when to use these methods to measure critical chemical and physical parameters in lube and hydraulic oils</li>
<li>What thermometric titration is and how it helps to improve robustness of serial acid number testing</li>
<li>How to automate measurements of large sample series to increase efficiency and reproducibility</li>
<li>How Vis-NIRS for lubricant analysis complies with ASTM guidelines and can save costs and time</li>
</ul>
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<p><strong>Presenter:</strong> Robert Tobler (Application Specialist Titration)</p>
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<p>Robert Tobler studied chemistry at the Zurich University of Applied Science in Wädenswil, Switzerland. He joined Metrohm in 2015 as an application specialist titration. Since 2018 he has also been involved in stability measurement.</p>
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<p><strong>Presenter:</strong> Nicolas Rühl, PhD (Product Manager Spectroscopy)</p>
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<p>Dr. Nicolas Rühl is working in the Competence Center Spectroscopy since 2015. In this time, he has been in charge of developing the near-infrared (NIR) spectroscopy business at Metrohm. Nicolas has a Master in Business Administration and received his PhD in physical chemistry on single-molecule spectroscopy using Vis-NIR and Raman spectroscopy technologies.</p>
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<p><strong>Moderator:</strong> Tom Lynch CChem FRSC (Independent Analytical Consultant, International Labmate)</p>
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<p>Tom has 35 years of experience in the petroleum industry, specialising in the delivery of forensic and problem solving /method development capabilities for BP Fuels and Lubricants businesses globally. He was also a member of the BP Science Council representing Analytical Science and led a BP wide Analytical Science network.Tom has published over 30 citable papers, 4 book chapters and has given over 60 presentations at conferences. He is a past Vice President of the Royal Society of Chemistry (RSC) Analytical Division and a past Chairman of the RSC Separation Science Group. In addition, Tom is a recipient of the Silver Jubilee Medal by the Chromatographic Society and a technical achievement award by the Energy Institute.</p>
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Metrohm: Making your quality control of hydraulic and lube oils more efficient

Making your quality control of hydraulic and lube oils more efficient

Combining titration and Vis-Near-Infrared Spectroscopy for higher sample throughput & faster results

Efficient quality control of hydraulic and lube oils

<p><strong>Traditional LC-based metabolomics workflows give maximum depth of information for bulk materials and are critical for untargeted profiling. Additional spatial information is possible with the versatility of the timsTOF platform and enables you to do both traditional and spatial workflows on the same instrument. MALDI Imaging is a true multiomics technique that allows for spatial determination of metabolites and lipids in tissue, as well as expanding targeted analysis to glycans and proteins. The addition of microGRID technology enables true 5 micron imaging for maximum biological information gain.</strong></p>
<h5>What to expect?</h5>
<ul>
<li><strong>Live presentation from Bruker’s Billerica demo facility to see ease of use of the timsTOF fleX platform</strong></li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Scientists and researchers interested in expanding their metabolomics workflows to the spatial dimension, scientists working with spatial distribution of small molecule biologics </li>
<li>MALDI Imaging users wanting to expand their workflows to small molecules</li>
<li>Anyone interested in learning more about MALDI Imaging capabilities</li>
</ul>
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<p><strong>Presenter:</strong> Kate Stumpo, Ph.D. (Business Development Manager, SpatialOMx, Bruker Scientific, LLC, Billerica, MA, USA)</p>
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<p><strong>Presenter:</strong> Josh Fischer, Ph.D. (Applications Scientist, Imaging, Bruker Scientific, LLC, Billerica, MA, USA)</p>
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<p><strong>Presenter:</strong> Ethan Yang, Ph.D. (Applications Lead, Imaging, Bruker Scientific, LLC, Billerica, MA, USA)</p>
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Bruker: Expanding to the spatial dimension in metabolomics with MALDI Imaging Mass Spectrometry

Expanding to the spatial dimension in metabolomics with MALDI Imaging Mass Spectrometry

Live presentation from Bruker’s Billerica demo facility to see ease of use of the timsTOF fleX platform

Metabolomics with MALDI Imaging Mass Spectrometry

<p><strong>Over the last two and a half years, we’ve all had to learn to communicate and work in new ways. With the help of technology, we’ve connected with colleagues across the globe in ways we never thought possible.  The ability to work remotely has ensured continued quality management of products consumed daily. For an analytical chemist or lab manager, quality management may include reviewing and approving data from the safety of your office whether in the lab or at home. No matter where the data is reviewed, completeness, accuracy and traceability must be maintained. This can be accomplished with a secure and efficient software platform.</strong></p>
<p>During this webinar, Kerri-Ann Blake, Product Manager for Titration, and Daniel Schmidt, IT Systems Engineer, will introduce you to the OMNIS data management platform. They will discuss how you can securely implement the software, review and sign data. You’ll learn how to track it all via audit trail from anywhere in the world - giving you peace-of-mind that all your data is being kept safe. </p>
<h5>Key Learning Objectives</h5>
<ul>
<li><strong>Introduce OMNIS software’s seamless networking capability and user management system</strong></li>
<li><strong>Demonstrate convenient audit trail features with next level compliance and data integrity</strong></li>
<li><strong>Discuss system security and ease of implementation</strong></li>
</ul>
<h5>Who Should Attend</h5>
<ul>
<li>QC/QA, R&amp;D and Process Development Lab Managers and Users </li>
<li>Anyone performing titrations in a 21 CFR Part 11 Compliant Environment </li>
<li>Those interested in data security and integrity</li>
</ul>
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<p><strong>Presenter:</strong> Kerri-Ann Blake (Titration Product Manager, Metrohm USA)</p>
</blockquote>
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<p><strong>Presenter:</strong> Daniel Schmidt (IT Systems Engineer, Metrohm USA)</p>
</blockquote>
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<p><strong>Presenter:</strong> Catherine Dold (Health &amp; Environment Writer, C&amp;EN Media Group)</p>
</blockquote>


C&EN: Peace-of-Mind with Secure Data Management

Peace-of-Mind with Secure Data Management

Kerri-Ann Blake, Product Manager for Titration, and Daniel Schmidt, IT Systems Engineer, will introduce you to the OMNIS data management platform.