In today’s academic research environment, speed and precision are everything. Whether you're analyzing trace organic compounds, processing environmental extracts, or running a high-throughput metabolomics project, one bottleneck continues to slow down even the most well-equipped labs: solvent evaporation.If your lab still relies on aging hot plates or hand-drying methods, you’re not alone. But the consequences are real: inconsistent recovery, lost analytes, and valuable hours wasted each week.<h3>The Hidden Cost of Manual Evaporation</h3>Many academic labs were built on traditional sample prep methods. But as research needs evolve and sample volumes increase, these outdated tools can no longer keep up.Manual evaporation doesn’t just slow workflows—it undermines reproducibility.Hot plates can over-dry samples or introduce uneven heating that degrades sensitive compounds. Open evaporation risks contamination. And when multiple students share equipment, timing differences from sample to sample introduce variability that shows up later as noisy chromatography or inconsistent quantitation.Students and faculty alike often report frustration with drying steps taking far too long, especially when preparing dozens of samples for <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis?hsLang=en">LC-MS</a> or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis?hsLang=en">GC-MS workflows</a>. In some cases, samples left unattended can be partially lost or require re-preparation entirely.For grant-funded research, that wasted time and material adds up quickly.<h3>A Better Way: Controlled Nitrogen Blowdown</h3>Nitrogen blowdown evaporation replaces guesswork with control. A steady stream of inert nitrogen combined with gentle, regulated heat speeds evaporation while protecting sensitive analytes.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=362&amp;manufacturerItems=540">Systems from Organomation </a>are designed specifically for sample preparation—not as improvised solutions or general lab accessories, but as purpose-built tools for consistent concentration.The result is straightforward:<ul style="list-style-type:disc;"><li>Faster dry-downs</li><li>Better recoveries</li><li>Improved reproducibility</li><li>Less hands-on time</li></ul>So researchers can focus on generating data instead of babysitting samples.<h3>Why Academic Labs Choose the N-EVAP</h3>Research labs rarely process identical samples all day.One student may be concentrating a small organic extract, another working with aqueous environmental samples, and a third reducing a larger crude matrix—all at the same time. That variability is exactly where many batch systems fall short.The<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=362&amp;manufacturerItems=540&amp;search=n-evap"> N-EVAP</a> transforms sample preparation uncertainty into control through radical flexibility.Unlike systems that force you to work around equipment constraints, the N-EVAP’s individual per-sample valves let you adjust gas flow, temperature exposure, and needle positioning for each sample—simultaneously.This isn’t theoretical flexibility. It means you can:<ul><li>Concentrate a 10 mL aqueous sample</li><li>Reduce a 5 mL organic extract</li><li>Evaporate a 50 mL environmental sample</li></ul>—all in the same run, without compromise.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/manual-to-automated-solvent-drying-is-it-worth-the-switch">Learn how the University of Cincinnati achieved 20x faster sample prep with the N-EVAP evaporator.</a></blockquote><ul><li>For academic programs handling variable matrices, this eliminates the false choice between batch efficiency and analytical precision. Each sample gets exactly the conditions it needs, preserving analyte integrity while maintaining throughput. &nbsp;</li><li>Available in configurations from <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2352">6</a> to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1911">45 </a>positions—with optional digital controls on larger models—the system scales easily from small teaching labs to large research groups without forcing workflow changes. &nbsp;</li><li>It’s built for scientists who want control over their experiments, not workarounds. &nbsp;</li><li>Our evaporators are the <a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/why-organomation-is-the-most-cited-blowdown-evaporator-brand-in-u.s.-epa-methods">most cited nitrogen blowdown sample concentrators</a> in standardized EPA methods, making Organomation the trusted, fail proof choice.</li></ul><h3>Built for Chromatography Workflows</h3>Since 1959, Organomation has focused exclusively on sample preparation. That specialization shows in the details that matter most to chromatography labs: uniform heating, consistent gas distribution, compatibility with common vial formats, and rugged construction that stands up to daily use in shared academic spaces.Whether you're preparing samples for LC-MS, GC-MS, or other analytical methods, nitrogen blowdown helps ensure:<ul><li>Faster turnaround between experiments</li><li>Consistent recoveries</li><li>Fewer re-runs</li><li>Less student labor tied up in drying steps</li></ul>Which ultimately means more time spent on analysis, interpretation, and publication.<h3>Prove the Time Savings for Yourself</h3>Upgrading your evaporation workflow can clearly improve efficiency—but how much time would your lab actually save? ➔ Rather than guessing, you can calculate it.Organomation’s free <a target="_blank" rel="noopener noreferrer" href="https://explore.organomation.com/evaporation-time-calculator-excel-download">Evaporation Time Savings Calculator</a> lets you enter your real lab conditions—sample counts, volumes, current dry-down times, and run frequency—to quantify exactly how much time nitrogen blowdown could save each week, month, or semester.For many academic labs, the results are eye-opening:<ul><li>Hours of student and staff time reclaimed</li><li>Faster LC-MS/GC-MS queues</li><li>Reduced bottlenecks before analysis</li><li>Hard numbers that help justify equipment purchases in grants and budgets</li></ul>In just a few minutes, you’ll have concrete data showing whether flexible evaporation makes financial and workflow sense for your lab.<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://explore.organomation.com/evaporation-time-calculator-excel-download">Download the Evaporation Time Savings Calculator and see what your lab could save.</a></blockquote>

Is Your University Lab Losing Time and Data in Sample Prep? Here’s How to Fix It

<h4>The science behind gummy candies</h4>Who can resist colourful, fruity gummy candies 🍬 with their signature chewy and elastic texture? While they may appear simple, their formulation is actually quite sophisticated. Each gummy is produced using a precisely balanced combination of gelling agents, sugars, acids, flavourings, and colourants, all working together to deliver the desired taste and texture.For instance, the characteristic chewiness of gummy sweets results from the interaction between gelatin and sugars. Even minor adjustments—such as changing the type or concentration of the gelling agent—can significantly alter the final product. In addition, manufacturers must meet regulatory and labeling requirements, including certifications like Halal compliance. This makes accurate and consistent monitoring of ingredients essential throughout production.<h4>The importance of SEC fingerprinting</h4>Size Exclusion Chromatography (SEC), also known as Gel Permeation Chromatography (GPC), offers a fast and reliable way to analyze gummy formulations. By separating molecules according to their hydrodynamic size, SEC generates a unique molecular “fingerprint” for each product.This fingerprint enables manufacturers to:<ul><li>confirm the type and origin of gelling agents (e.g., for Halal verification)</li><li>ensure batch-to-batch consistency and detect production variations</li><li>monitor changes during storage to assess shelf life</li><li>evaluate low-molecular-weight compounds such as sugars in a semi-quantitative manner</li></ul>Overall, SEC fingerprinting supports consistent product quality and regulatory compliance, ensuring that every gummy maintains the same expected characteristics.<h4>Influence of column selection on SEC performance</h4>Column selection plays a crucial role in achieving high-quality separations and detailed molecular fingerprints. To illustrate this, porcine and bovine gelatin standards were analyzed using two different configurations:<ul><li>a single Multipore <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1655">SuperOH-P column</a></li><li>a coupled system of SuperOH-P 350 and SuperOH-P 150 columns</li></ul>Although both setups cover the same molecular weight range (100–1,000,000 Da), the coupled configuration effectively doubles the separation length. This results in improved resolution, which was confirmed experimentally. The coupled columns provided clearer separation between porcine and bovine gelatin, producing more distinct and informative fingerprints.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_1_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_d81181cfb4.jpg" alt="KNAUER: Fig. 1 Overlay chromatograms measured with DAD at 230 nm, 20 µL. Dark blue: porcine reference standard; red: bovine reference standard; solid line: measured with column A; dotted line: measured with column set B. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_1_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_d81181cfb4.jpg 245w," sizes="100vw" width="1024" height="611"><h4>Achieving reliable SEC fingerprints</h4>Reproducibility is key when using SEC for quality control. The process begins with consistent sample preparation—maintaining identical dissolution procedures, concentrations, and filtration steps is essential, as even small variations can influence peak shape and overall results.Beyond the column, the entire system setup is equally important. For example, combining a Refractive Index Detector (RID) with a Diode Array Detector (DAD) allows simultaneous detection of sugars, acids, and polymers within a single analysis.Accurate peak identification further enhances reliability. By analyzing reference standards based on known ingredient compositions, chromatographic peaks can be correctly assigned, enabling detailed interpretation of both high- and low-molecular-weight components.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_2_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_37caf5ac59.jpg" alt="KNAUER: Fig. 2 Zoom overlay chromatograms measured with RID, 20 µL and column set B. Black: Katjes® Yoghurt Gums; turquoise: apple pectin diluted 1:10; dark green: preserving sugar 1:1 (sugar; citric acid; pectin; fully hydrogenated sunflower oil); dark blue: maltodextrin; pink: sodium potassium tartrate; yellow: potassium citrate; red: citric acid; purple: lactic acid; beige: lactose; orange: sucrose; green: glucose; grey: blank. Graphic by KNAUER. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_2_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_37caf5ac59.jpg 245w," sizes="100vw" width="1024" height="611">Additionally, incorporating a flow marker helps correct for system-related variations, such as fluctuations in flow rate, thereby improving retention time stability and peak assignment accuracy. More details on the flow marker and its use in SEC are described in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0043_knauer_gpc_sec_data_reproducibility_with_flow_marker_96ee6b3cd9.pdf">this technical note</a>.<h4>Verification of gelatin origin using SEC</h4>Determining the source of gelatin is critical for proper labeling and compliance, particularly for Halal-certified products where porcine gelatin is not permitted.To demonstrate SEC’s capability, two gummy samples with identical formulations but different gelatin sources were analyzed. One sample contained porcine gelatin, while the other used bovine gelatin. The resulting SEC fingerprints showed clear differences in the gelatin region, allowing unambiguous identification. The standard Haribo® Goldbears corresponded to the porcine reference, whereas the Halal version matched the bovine standard.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_3_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_and_column_set_B_12e99a43e7.jpg" alt="KNAUER: Fig. 3 Overlay chromatograms measured with DAD at 230 nm, 20 µL and column set B. Solid dark blue line: Haribo® Goldbears; solid red line: Halal Haribo® Goldbears; dotted dark blue line: porcine reference standard; dotted red line: bovine reference standard. Graphic by KNAUER. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_3_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_and_column_set_B_12e99a43e7.jpg 245w," sizes="100vw" width="1024" height="611"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_2_c29ea59bc4.jpg" alt="KNAUER: Fig. 4 Overlay chromatograms measured with RID, 20 µL and column set B. Solid dark blue line: Haribo® Goldbears; solid red line: Halal Haribo® Goldbears; dotted dark blue line: porcine reference standard; dotted red line: bovine reference standard. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_2_c29ea59bc4.jpg 245w," sizes="100vw" width="1368" height="432">This confirms that SEC is a reliable method for distinguishing gelatin origin.<h4>Role of low-molecular-weight components</h4>In addition to polymers such as gelatin, SEC can also reveal smaller molecules, including sugars and organic acids. While SEC is not a replacement for fully validated HPLC methods, it serves as a rapid screening tool for comparing samples and supporting quality control.Using RID detection, differences in sugar composition become visible. For example, chromatograms showed a significantly higher sucrose signal in preserving sugar compared to gummy candies, reflecting formulation differences.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_5_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_06c072e069.jpg" alt="KNAUER: Fig. 5 Zoom overlay chromatograms measured with RID, 20 µL and column set B. Black: sucrose 1mg/ml; blue: Haribo® Goldbears; green: preserving sugar 1:1 (sugar; citric acid; pectin; fully hydrogenated sunflower oil). Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_5_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_06c072e069.jpg 245w," sizes="100vw" width="1024" height="611"><h4>Conclusion</h4>SEC is a powerful analytical technique for monitoring product quality and ensuring process consistency in gummy candy production. By generating detailed molecular fingerprints, it enables manufacturers to verify ingredients, maintain consistency, and comply with regulatory requirements.Importantly, this approach is not limited to gummy candies—it can be adapted to a wide range of products where molecular characterization is essential.<blockquote><ul><li>If you're planning to set up or optimize your GPC/SEC workflow, feel free to contact us at sales@knauer.net. Stay tuned for more exciting insights into the GPC/SEC world in our “GPC/SEC Made Easy” series.</li><li>For more information on the technical details, you can refer to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VFD_0195_sec_for_fingerprint_analysis_of_gummy_candies_ce5349cd95.pdf">this application note.</a></li><li>For further information on this topic, please contact our author: wesolowski@knauer.net</li></ul></blockquote>

GPC/SEC Made Easy – Fingerprint analysis of Gummy Candies

News from LabRulezLCMS Library - Week 17, 2026

Article

Product

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Food

Science and research

Laboratories

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Separation Science

Agilent Technologies

Shimadzu Corporation

<ol>
<li>Strategies for sample cleanup</li>
<li>Chromatography problems caused by sample matrix</li>
</ol>
<ul>
<li>Physical effects</li>
<li>Chemical effects</li>
</ul>

Agilent Technologies: Techniques for Avoiding Unexpected Problems in LC and GC Analysis

Techniques for Avoiding Unexpected Problems in LC and GC Analysis

<h3>This presentation will cover:</h3>
<ul>
<li>Some introspection</li>
<li>How you view your analytical system</li>
<li>MS and MS/MS 101</li>
<li>Anatomy of an LC system</li>
<li>Is mass spec &#39;just a detector&#39;?</li>
<li>Anatomy of an LC-MS(MS) system</li>
<li>Low vs High resolution method development</li>
<li>Advantages and disadvantages of LC-MS(MS)</li>
<li>HPLC vs LC-MS(MS) method development</li>
</ul>
<blockquote>
Presenter: Stephen Brookes (LC/MS Technical Sales Specialist, Shimadzu UK Limited)
</blockquote>

Shimadzu: LC-MS(MS) Method Development: HPLC vs LC-MS(MS) Method Development

LC-MS(MS) Method Development: HPLC vs LC-MS(MS) Method Development

Nitrosamine compounds are classified as probable human carcinogens by the International Agency for Research on Cancer (IARC). Ever since the first U.S. Food and Drug Administration (FDA) notification in June 2018 about levels of these impurities in pharmaceutical API, they have been a reason of continuous discussion globally for analytical scientist, patients and regulators.
After June 2018, the number of recalls and 483’s have been on a rise for not only angiostenin II receptor blockers (-ARB, also called as sartans) but also for other products like Ranitidine, Metformin etc. FDA, European Medicine Agency (EMA) and International Council for Harmonisation (ICH) have constantly set and update the guidelines in measuring impurities in pharmaceutical products.
<h3>Q&amp;A Panel Discussion along with our Expert Panelists:</h3>
<ul>
<li>Dr. Mrunal Jaywant (Senior Director – R&amp;D, USP India)
</li>
<li>Pravin Makwana (General Manager - QC, J.B.Chemicals &amp; Pharmaceuticals Ltd.)
</li>
<li>Nilesh Dhamorikar (Director, QbD Research and Development Lab Pvt Ltd)
</li>
</ul>
<blockquote>
Presenter: Dheeraj Handique (Deputy Manager-CSC, Shimadzu Analytical (India) Pvt. Ltd)
</blockquote>
<blockquote>
Dheeraj Handique is working as Deputy Manager-CSC at Shimadzu Analytical (India) Pvt. Ltd. since 2008. He holds a Post-Graduate Diploma in Analytical Chemistry from Bombay Institute of Technology, Mumbai, with total experience of 17 years in analytical field industries.
</blockquote>
<blockquote>
At Shimadzu, his role involves pre-sales and post-sales application support to pharma, chemical, food safety and flavor and fragrance industry customers pertaining to trouble shooting, tech transfer, method development and validation. His key areas are to suggest complete system configuration with total solution related to GC, GCMS/MS instruments to customers. To his credit he has around 30 posters published in international conferences.
</blockquote>
<blockquote>
His expertise lies in analysis of drug substances and products, various food matrices, flavor and fragrance matrices with cutting edge technology like GCMS/GCMSMS/HS/TD from Shimadzu with hands on experience on critical sample preparation steps involved. He delivers lectures on Gas chromatography and mass spectrometry, headspace and thermal desorption techniques at various national conferences.
</blockquote>
<blockquote>
Also, he has been very actively involved in the performance evaluation of various chromatographic and advanced technologies available with Shimadzu like GC, GCMS, GCMSMS, HS, TD etc.
</blockquote>
<blockquote>
Presenter: Shailendra Rane (Ph.D.) (Deputy Manager-CSC, Shimadzu Analytical (India) Pvt. Ltd)
</blockquote>
<blockquote>
Dr. Shailendra Rane is Ph.D. in chemistry from Mumbai university and currently working with Shimadzu Analytical (India) Pvt. Ltd. as Deputy Manager CSC since 2008. At Shimadzu, his role involves pre- and post-sales application support to pharma, food safety and bio-similar customers pertaining to trouble shooting, tech transfer, method development and validation. To his credit he has 10 publications in various international journals of repute and around 35 posters he published in national and international conferences.
</blockquote>
<blockquote>
His expertise lies in analysis of drug substances and products, various food matrices and bio-molecules with cutting edge technology like LCMS/LCMSMS from Shimadzu with hands on experience on critical sample preparation steps involved. He delivers lectures on liquid chromatography and mass spectrometry at various national conferences. Also, he has been very actively involved in the performance evaluation of various chromatographic and advanced technologies available with Shimadzu like SFC, MALDI-ToF etc.
</blockquote>
<blockquote>
Presenter: Rashi Kochhar Rebello (Manager, Marketing Communications, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Rashi has been part of Shimadzu for 13 years now. She has worked extensively with Chromatographic and MS solutions in different capacities. Her expertise lies in supporting workflows for different markets and segments including Biopharma, Pharma and Food. With this background, her current role is to design communication packages for analytical solutions in Shimadzu Asia Pacific.
</blockquote>

Shimadzu: Confidently Analyse Nitrosamine Impurities by GC-MS/MS & LC-MS/MS

Confidently Analyse Nitrosamine Impurities by GC-MS/MS & LC-MS/MS

There are many variables to consider during method development, some more obvious than others. Laying the groundwork early with clear goals will help you develop robust and rugged methods that are easier to use, validate, and transfer.
In this presentation, we will look at:
<ul>
<li>How to assess the effect of varying a parameter
</li>
<li>Adjustments you can make to address variability
</li>
<li>Chemistry and instrument configuration factors that can affect the robustness and ruggedness of HPLC separations
</li>
<li>A rugged, robust method is not only cost effective in the long term, but it also increases the likelihood of having a method that will pass with flying colors when you transfer it to other in-house or contract analytical laboratories.
</li>
</ul>
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: Conquer Method Variability: Evaluate Variables During Method Development

Conquer Method Variability: Evaluate Variables During Method Development

Laying the groundwork early with clear goals will help you develop robust and rugged methods that are easier to use, validate, and transfer.

Evaluate Variables During HPLC Method Development

Good resolution is necessary for accurate identification and quantitation of your analytes but how much is enough?
In this seminar we’ll look at:
<ul>
<li>Strategies for getting the resolution you need
</li>
<li>HPLC parameters that are key contributors to resolution
</li>
<li>What to consider when using a gradient
</li>
<li>The role of the instrument
</li>
</ul>
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

Agilent Technologies: HPLC Resolution: Too Much, Too Little, or Just Right?

HPLC Resolution: Too Much, Too Little, or Just Right?

Good resolution is necessary for accurate identification and quantitation of your analytes but how much is enough?

Improving an existing method is an opportunity to save time, money and sample.
Whether you’re developing a new method or updating an older one, there are several questions to consider, including:
<ul>
<li>Is the goal to reduce analysis time and/or improve resolution?
</li>
<li>Is the method isocratic or gradient?
</li>
<li>What HPLC parameters will be key to success?
</li>
<li>What are the column options and will they work with your instrumentation?
</li>
<li>Modernizing a legacy method, whether it’s a reference method from the USP, AOAC, or EPA or an internal one, can simplify testing and improve accuracy, ruggedness, and sample throughput.
</li>
</ul>
<blockquote>
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
</blockquote>

Agilent Technologies: Updating Old Methods, Is the Gain Worth the Pain?

Updating Old Methods, Is the Gain Worth the Pain?

Improving an existing method is an opportunity to save time, money and sample.

A short review of GPC/SEC theory and technique including conventional and multiple detection analysis is followed by a look at current applications of GPC/SEC in the fields of Biotechnology and Biopharmaceuticals research and manufacturing.
GPC/SEC has traditionally been applied in synthetic polymer production and related product manufacturing. It has also been widely used in analysis of natural products. GPC/SEC provides valuable characterization by molecular size, molecular weight and molecular weight distribution. It is now being increasing applied to discovery, production and manufacturing of materials for Biotechnology and Biopharmaceuticals in drug deliveries, therapeutic products and medical devices. Attendees will benefit from review of technique and applications information.
<blockquote>
Presenter: Richard J. Lentner (Field Applications Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Richard J. Lentner is a Field Applications Engineer with Agilent Technologies supporting the broad portfolio of Agilent products for GPC including hardware systems, specialty systems (HTGPC, Triple Detection GPC) Software (GPC/SEC, Multi Detection GPC, BIO-SEC), GPC consumable GPC Columns and Standards. He delivers training and consulting within America Field Operations with over thirty years in GPC support, sales and service of GPC related products. His studies include Biology, Chemistry and Biochemistry.
</blockquote>

Agilent Technologies: Applications in Gel Permeation/Size Exclusion Chromatography (GPC/SEC) for the Biotechnology and Biopharmaceutic

Applications in Gel Permeation/Size Exclusion Chromatography (GPC/SEC) for the Biotechnology and Biopharmaceutic

GPC/SEC theory and technique including conventional and multiple detection analysis.

Applications in Gel Permeation/Size Exclusion Chromatography

If you’ve ever looked at a chromatogram and said, “What’s that?” or thought “That’s not supposed to be there!”, this talk is for you.
We will be discussing:
<ul>
<li>Chromatography problems that can be caused by sample matrix
</li>
<li>How to deal with unwanted matrix effects
</li>
<li>Strategies for cleaning up your sample and how that helps protect your column and instrument
</li>
<li>Unexpected sources of unknown peaks
</li>
<li>Unexpected peaks and issues are often the result of the sample matrix. Understanding what to do about it can help you get the reliable and reproducible chromatography you want.
</li>
</ul>
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
</blockquote>
<blockquote>
Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>

Agilent Technologies: Oh, What a Mess! Dealing with Unwanted Matrix Effects

Oh, What a Mess! Dealing with Unwanted Matrix Effects

If you’ve ever looked at a chromatogram and said, “What’s that?” or thought “That’s not supposed to be there!”, this talk is for you.

Whether you’re starting out working on biomolecules or transitioning from small molecules to biotherapeutics you will find some attributes can make biomolecule separations more challenging.
This presentation will look at what we typically think about for small molecules and how working with biomolecules may be similar or in some cases quite different.
We’ll discuss:
<ul>
<li>Method development parameters; similarities and differences
</li>
<li>Column selection
</li>
<li>The matrix and sample prep
</li>
<li>Instrument considerations
</li>
<li>Tips to help identify why your separation doesn’t look like you thought it would
</li>
<li>Although the focus of this talk is reversed phase, we will also review other types of chromatography used for biomolecules including HIC, SEC, and IEX.
</li>
</ul>
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: Entering the Bio Zone: Reversed Phase from Small Molecules to Biomolecules

Entering the Bio Zone: Reversed Phase HPLC from Small Molecules to Biomolecules

What we typically think about for small molecules and how working with biomolecules may be similar or in some cases quite different.

Reversed Phase HPLC from Small Molecules to Biomolecules

The increasing complexity of the food supply chain, the exponential growth of chemical hazards and their regulations have brought several challenges to the field of food safety.
The need for productive gains and increased confidence in reporting non-compliant samples are particularly noteworthy challenges for laboratories that are routinely relied upon for maintaining a safe food supply chain. To meet these demands, mass spectrometric techniques such as tandem mass spectrometry and quadruple-time-of-flight mass spectrometry have emerged as the detectors of choice for the analysis of organic chemical hazards in foods and beverages.
One of the more recent significant developments is Shimadzu’s ultra fast family of mass spectrometric detectors that provide opportunities to increase both productivity and reporting confidence compared with more conventional mass spectrometric detectors. The importance of these state-of-the-art instruments to the analysis of chemical hazards in food will be discussed in the context of the regulatory requirements for the analysis of contaminants in foods and a general overview of chemical hazards in foods.
By attending this webinar you will:
<ul>
<li>Understand the core challenges for food testing
</li>
<li>Recognize the key elements that can accelerate and streamline your workflow and increase your data confidence
</li>
<li>Learn and apply in various food safety analysis (e.g. multi-residual pesticides, veterinary drugs and mycotoxins).
</li>
</ul>
Who should attend?
<ul>
<li>Scientists and lab personnel conducting food analysis
</li>
<li>Lab managers in testing labs, regulatory agencies and the food industry
</li>
<li>Everyone who is keen to increase their lab productivity and data confidence for food and beverage analysis.
</li>
</ul>
<blockquote>
Presenter: Dr Peter Varelis (Applications Manager, Shimadzu Scientific Instruments (Oceania))
</blockquote>
<blockquote>
Dr Peter Varelis has more than 20 years of research experience in both government and industry. He was also on an Elsevier editorial board (Food Chemistry and Analysis). His current research interest is the application of mass spectrometry to investigate organic compounds that have implications for human health and nutrition.
</blockquote>

Shimadzu: Ultra Fast Mass Spectrometry for the Analysis of Chemical Hazards in Food & Beverages

Ultra Fast Mass Spectrometry for the Analysis of Chemical Hazards in Food & Beverages

How the use of ultra fast spectrometry can help overcome some of these challenges by highlighting the importance of the technique for the analysis.